PDF DatasheetRat TLR4 / CD284 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TLR4 / CD284 based upon sequence from Rat
Host: QP9988 protein expressed in Invitro-E.coli.
Tag: HIs-Myc
Protein Construction: A DNA sequence encoding the Rattus norvegicus (Rat) TLR4 / CD284, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP9988.
Bioactivity Data: Untested
Full Length? Extracellular Domain
Expression Region: Asn26 – Ser638
Amino Acid Sequence: NPCIEVLPNI TYQCMDQNLS KIPHDIPYST KNLDLSFNPL KILRSYSFTN FSQLQWLDLS RCEIETIEDK AWHGLNQLST LVLTGNPIKS FSPGSFSGLT NLENLVAVET KMTSLEGFHI GQLISLKKLN VAHNLIHSFK LPEYFSNLTN LEHVDLSYNY IQTISVKDLQ FLRENPQVNL SLDLSLNPID SIQAQAFQGI RLHELTLRSN FNSSNVLKMC LQNMTGLHVH RLILGEFKNE RNLESFDRSV MEGLCNVSID EFRLTYINHF SDDIYNLNCL ANISAMSFTG VHIKHIADVP RHFKWQSLSI IRCHLKPFPK LSLPFLKSWT LTTNREDISF GQLALPSLRY LDLSRNAMSF RGCCSYSDFG TNNLKYLDLS FNGVILMSAN FMGLEELEYL DFQHSTLKKV TEFSVFLSLE KLLYLDISYT NTKIDFDGIF LGLISLNTLK MAGNSFKDNT LSNVFTNTTN LTFLDLSKCQ LEQISRGVFD TLYRLQLLNM SHNNLLFLDP SHYKQLYSLR TLDCSFNRIE TSKGILQHFP KSLAVFNLTN NSVACICEYQ NFLQWVKDQK MFLVNVEQMK CASPIDMKAS LVLDFTNSTC YIYKTIISVS VVS
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Rat TLR4 / CD284 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Rat TLR4 / CD284 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tlr4 |
| Entrez Gene ID | 29260 |
| RefSeq Protein Accession(s) | NP_062051.1 |
| RefSeq mRNA Accession(s) | NM_019178.1 |
| UniProt ID(s) | Q9QX05 |
| UniGene ID(s) | Rn.14534 |
| COSMIC ID Link(s) | Tlr4 |
| KEGG Gene ID(s) | rno:29260 |
| General Description of Recombinant Rat TLR4 / CD284 Protein. | |
| Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.