PDF DatasheetTritirachium album Protein K Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PROK based upon sequence from Tritirachium album Limber
Host: QP15080 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PROK was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP15080.
Bioactivity Data: Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated ＞30U/mg.
Amino Acid Sequence:
MRLSVLLSLL PLALGAPAVE QRSEAAPLIE ARGEMVANKY IVKFKEGSAL SALDAAMEKI SGKPDHVYKN VFSGFAATLD ENMVRVLRAH PDVEYIEQDA VVTINAAQTN APWGLARISS TSPGTSTYYY DESAGQGSCV YVIDTGIEAS HPEFEGRAQM VKTYYYSSRD GNGHGTHCAG TVGSRTYGVA KKTQLFGVKV LDDNGSGQYS TIIAGMDFVA SDKNNRNCPK GVVASLSLGG GYSSSVNSAA ARLQSSGVMV AVAAGNNNAD ARNYSPASEP SVCTVGASDR YDRRSSFSNY GSVLDIFGPG TSILSTWIGG STRSISGTSM ATPHVAGLAA YLMTLGKTTA ASACRYIADT ANKGDLSNIP FGTVNLLAYN NYQA
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: See the COA sent with the product.
Endotoxin Levels: < 50 EUs/mg. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PROK protein solution (lyophilized) containing Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human PROK General Information | |
|---|---|
| Alternate Names | |
| Endopeptidase K,Tritirachium alkaline proteinase | |
| Molecular Weight | |
| 28.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P06873 |
| General Description of Recombinant Human PROK. | |
| Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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