Human Anti-AMP Deaminase, Isoform E Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (1-2ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunocytochemistry (1-2.0ug of antibody/ml for 30 minutes at RT), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-AMP Deaminase, Isoform E Antibody Clone: AMPD3/901
Clone AMPD3/901 Host and Isotype: Mouse IgG2b kappa
Anti-Human AMP Deaminase, Isoform E Positive Control Sample: RBC. Fetal liver; Spleen or Placenta
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human AMDP3 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
AMP Deaminase, Isoform E Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-AMPD3 antibody staining is expected to be primarily localized to the nuclear membrane.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of AMP Deaminase, Isoform E expression as measured by anti-AMP Deaminase, Isoform E antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | – | – | + | – | – | – | + | – | – | – | – | – | – | – | – | – | + |
| AMPD3 Variability | + | ++ | + | ++ | + | ++ | + | + | + | +++ | + | + | ++ | ++ | + | + | + | + | ++ | ++ |
| AMP Deaminase, Isoform E General Information | |
|---|---|
| Alternate Names | |
| AMP deaminase 3, AMPD3 | |
| Molecular Weight | |
| ~90kDa | |
| Chromosomal Location | |
| 11p15.4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AMPD3 |
| Entrez Gene ID | 272 |
| Ensemble Gene ID | ENSG00000133805 |
| RefSeq Protein Accession(s) | NP_001020561, NP_001165901, NP_000471, NP_001020560, NP_001165902 |
| RefSeq mRNA Accession(s) | NM_000480, NM_001025390, NM_001172430, NM_001025389, NM_001172431, |
| RefSeq Genomic Accession(s) | NC_018922, NG_012041, NC_000011 |
| UniProt ID(s) | Q01432 |
| UniGene ID(s) | Q01432 |
| HGNC ID(s) | 470 |
| Cosmic ID(s) | AMPD3 |
| KEGG Gene ID(s) | hsa:272 |
| PharmGKB ID(s) | PA24778 |
| General Description of AMP Deaminase, Isoform E. | |
| It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids, is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M), liver (L) cells. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow,, fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. | |
Limitations and Warranty
enQuire Bio’s AMP Deaminase, Isoform E Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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