Anti-CD86 Antibody [PO3.1]

Name: Anti-CD86 Antibody [PO3.1]
Brand: enQuire Bio
Price: 59 USD
Availability: InStock

$ 59.00 – $ 169.00

Please Select Product Options Below To View The Catalog Number.

SKU: QAB93
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
QAB93-PE-25ug	Size: 25 ug, Tag: PE	$ 59.00
QAB93-PE-100ug	Size: 100 ug, Tag: PE	$ 169.00

Shipping Information

In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: QAB93-PE-25ug

CD86 General Information
Alternate Names
B7-2, B7.2
Curated Database and Bioinformatic Data
Gene Symbol	Cd86
Entrez Gene ID	12524
Ensemble Gene ID	ENSMUSG00000022901
RefSeq Protein Accession(s)	XP_011244114, XP_006521804, NP_062261
RefSeq mRNA Accession(s)	XM_011245812, XM_006521741, XR_384517, NM_019388
RefSeq Genomic Accession(s)	NC_000082
UniProt ID(s)	Q549Q9, Q61238, P42082
UniGene ID(s)	Q549Q9, Q61238, P42082
Cosmic ID(s)	Cd86
KEGG Gene ID(s)	mmu:12524
General Description of CD86.
The PO3.1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.The PO3.1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Mouse Anti-CD86 Antibody Product Attributes

Species: Mouse

Tested Applications: Flow Cytometry.

Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization

Clonality: Monoclonal Antibody

Anti-CD86 Antibody Clone: PO3.1

Clone PO3.1 Host and Isotype: Rat IgG2b kappa

Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2

Antibody Concentration: 0.2 mg/mL

Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD86 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	++	++	+	++	++	+	+	++	++	++	++	+	++	+	-	+	++	++	++	++
CD86 Variability	++	++	++	+	++	++	++	++	++	++	++	++	++	++	++	++	++	+	+	++

Selected References

Ioannou M, Alissafi T, Lazaridis I, Deraos G, Matsoukas J, Gravanis A, Mastorodemos V, Plaitakis A, Sharpe A, Boumpas D, and Vergiinis P. 2012. J. Immunol. 188: 1136-1146. (Flow cytometry)Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, and Azuma M. 2006. Int. Immunol. 18: 1375-1384. (Immunohistochemistry - frozen tissue)Kin NW and Sanders VM. 2006. J. Immunol. 176: 6727 - 6735. (in vitro activation)Spadaro M, Ambrosino E, Iezzi M, Di Carlo E, Sacchetti P, Curcio C, Amici A, Wei W-Z, Musiani P, Lollini P-L, Cavallo F, Forni G. 2005. Clin. Cancer Res. 11: 1941-1952. (Immunohistochemistry - frozen tissue)Pokojil JRIwai H, Kozono Y, Hirose S, Akiba H, Yagita H, Okumura K, Kohsaka H, Miyasaka N, and Azuma M. 2002. J. Immunol. 169: 4332-4339. (in vitro activation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD86 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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