PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-CD99 Antibody Clone: EP8
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Tonsil, Ewings Sarcoma
Cellular Localization of Antibody EP8 Staining: Membrane/Cytoplasm
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human CD99 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CD99 Information for Pathologists
| CD99 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 18.8 kDa | |
| Chromosomal Location | |
| p22.33 [chr: X] [chr_start: 2691187] [chr_end: 2741309] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD99 |
| Entrez Gene ID | 4267 |
| RefSeq Protein Accession(s) | NP_001308296; NP_002405; NP_001116370; NP_001308298; NP_001308297 |
| RefSeq mRNA Accession(s) | ; NM_001321368; NM_001321369; NM_001321370; NM_001122898; NM_002414; NM_001277710; NM_001321367; NR_135623 |
| RefSeq Genomic Accession(s) | NC_000023; NC_000024; NG_009174 |
| UniProt ID(s) | P14209 |
| PharmGKB ID(s) | PA30804 |
| KEGG Gene ID(s) | hsa:4267 |
| General Description of CD99 . | |
| CD99 is a transmembrane glycoprotein, also known as MIC2. It is involved in T cell adhesion, leukocyte migration and differentiation of primitive neuroectodermal cell. CD99 labels lymphocyte, ovarian granulosa cells, pancreatic islet cells, sertoli cells, CNS ependymal cells and endothelial cells. Antibody to CD99 has been useful in diagnosis of Ewing’s sarcoma, sex cord-stromal tumor, endocrine tumor of pancreas. Additionally, CD99 is found in a subset of other tumors including lymphoblastic lymphoma, breast carcinoma and other malignancies | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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