Human and Mouse (-) Anti-Androgen Receptor Antibody Product Attributes
Species: Human and Mouse (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Androgen Receptor Antibody Clone: DHTR/882
Clone DHTR/882 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse (-) Androgen Receptor Positive Control Sample: LNCap cells or Prostate carcinoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human DHTR protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Androgen Receptor Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-ar antibody staining is expected to be primarily localized to the mitochondria.Observed Antibody Staining Data By Tissue Type:
Variations in Androgen Receptor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the epididymis, seminal vesicle. More moderate antibody staining intensity was present in glandular cells in the epididymis, seminal vesicle. Low, but measureable presence of Androgen Receptor could be seen inglandular cells in the cervix, uterine. We were unable to detect Androgen Receptor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Androgen Receptor expression as measured by anti-Androgen Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | - | - | - | - | - | - | - | - | - | - | - | - | +++ | - | - | - | - | - | - |
AR Variability | ++ | + | + | + | ++ | + | ++ | + | + | + | + | ++ | + | + | + | ++ | + | + | + | + |
Limitations and Warranty
enQuire Bio's Androgen Receptor Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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