Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Calponin Antibody Clone: CALP (same as hCP)
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Uterus
Cellular Localization of Antibody CALP (same as hCP) Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Crude human uterus extract.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Calponin Information for Pathologists
Summary:
Actin filament associated regulatory protein. h1 (basic) isoform is smooth muscle specific, but a late stage smooth muscle marker; described below. h2 isoform is found in smooth muscle and non muscle cells; not described below. Uses by pathologists Identify myoepithelial cells in breast lesions (Histopathology 2005;47:202).Common Uses By Pathologists:
Identify myoepithelial cells in breast lesions (Histopathology 2005;47:202). Help differentiate breast collagenous spherulosis (positive) from adenoid cystic carcinoma (negative, Mod Pathol 2006;19:1351), although adenoid cystic carcinoma is calponin positive in salivary gland tumors (Arch Pathol Lab Med 1999;123:801). Microscopic (histologic) images Images hosted on Pathout server:.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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