Western Blot Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781).
Western Blot Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781).

Anti-Carbonic Anhydrase IX Antibody Clone CA9/781

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 768-MSM2
Species: Human and Horse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
768-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 768-MSM2-P1
Carbonic Anhydrase IX General Information
Alternate Names
Carbonic anhydrase 9, CA9, CAIX
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolCA9
Entrez Gene ID768
Ensemble Gene IDENSG00000107159
RefSeq Protein Accession(s)NP_001207
RefSeq mRNA Accession(s)XR_428428, NM_001216, XR_001746374
RefSeq Genomic Accession(s)NC_000009, NC_018920
UniProt ID(s)A0A0S2Z3D0, Q16790
UniGene ID(s)A0A0S2Z3D0, Q16790
HGNC ID(s)1383
Cosmic ID(s)CA9
KEGG Gene ID(s)hsa:768
PharmGKB ID(s)PA25998
General Description of Carbonic Anhydrase IX.
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, the formation of aqueous humor, cerebrospinal fluid, saliva, gastric juice. They show extensive diversity in distribution, in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.

Human and Horse Anti-Carbonic Anhydrase IX Antibody Product Attributes

Species: Human and Horse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Carbonic Anhydrase IX Antibody Clone: CA9/781
Clone CA9/781 Host and Isotype: Mouse IgG2b kappa
Anti-Human and Horse Carbonic Anhydrase IX Positive Control Sample: Normal kidney or renal cell carcinoma
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CA9 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Carbonic Anhydrase IX Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CA9 antibody staining is expected to be primarily localized to the plasma membrane and cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Carbonic Anhydrase IX antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the gallbladder, stomach. More moderate antibody staining intensity was present in glandular cells in the gallbladder, stomach. Low, but measureable presence of Carbonic Anhydrase IX could be seen in. We were unable to detect Carbonic Anhydrase IX in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Carbonic Anhydrase IX expression as measured by anti-Carbonic Anhydrase IX antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
CA9 Variability + + + + + + + + + + + + + + ++ + ++ + + ++

Limitations and Warranty

enQuire Bio's Carbonic Anhydrase IX Anti-Human, Horse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Carbonic Anhydrase IX Antibody Clone CA9/781