Human Anti-CD10 / Neprilysin / MME Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD10 / Neprilysin / MME Antibody Clone: SN5c
Clone SN5c Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD10 / Neprilysin / MME Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD10 / Neprilysin / MME antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, cells in the tubules in kidney and glandular cells in the duodenum, prostate and small intestine. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, cells in the tubules in kidney and glandular cells in the duodenum, prostate and small intestine. Low, but measureable presence of CD10 / Neprilysin / MME could be seen inmacrophages in lung. We were unable to detect CD10 / Neprilysin / MME in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP and Seon BK. 1987. Cancer Res. 47(8): 2160-2166. Shipp MA, Stefano GB, Switzer SN, Griffin JD and Reinherz EL. 1991. Blood. 78(7): 1834-1841. Pinho S, Lacombe J, Hanoun M, Mizoguchi T, Bruns I, Kunisaki Y and Frenette PS. 2013. J Exp Med. 201(7): 1351-1367. (Flow cytometry) Mukhopadhyay C, Zhao X, Maroni D, Band V and Naramura M. 2013. PLoS One. 8(10): e75907. (Flow cytometry)
Limitations and Warranty
enQuire Bio's Human Anti-CD10 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.