C57Bl/6 bone marrow cells were stained with FITC Mouse Anti-CD45R  and 0.06 ug APC Mouse Anti-CD117 (QAB54) (right panel) or 0.06 ug  APC Rat IgG2b (left panel).

Mouse Anti-CD117 / c-Kit Antibody [ACK2]

$ 49.00$ 269.00
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SKU: QAB54
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE ;Unconjugated ;APC ;APC-Cy7 ;PE-Cy7
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB54-PE-25ug
CD117 / c-Kit General Information
Alternate Names
CD117, c-Kit, Gsfsco1, Tr-kit, SOW3, Ssm, Bs, Gsfsow3, SCO1, SCO5, CD117, c-KIT, Fdc, Gsfsco5, W
Curated Database and Bioinformatic Data
Gene SymbolKit
Entrez Gene ID16590
Ensemble Gene IDENSMUSG00000005672
RefSeq Protein Accession(s)NP_001116205, XP_017176176, NP_066922
RefSeq mRNA Accession(s)XM_017320687, NM_021099, NM_001122733
RefSeq Genomic Accession(s)NC_000071
UniProt ID(s)P05532
UniGene ID(s)P05532
Cosmic ID(s)Kit
KEGG Gene ID(s)mmu:16590
General Description of CD117 / c-Kit.
The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo (use format appropriate for functional assays). In addition, the antibody is reported to be cross-reactive with rat c-Kit and is extensively published for use in this species.

Mouse Anti-CD117 / c-Kit Antibody Product Attributes

Species: Mouse
Cross Reactivity: rat
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD117 / c-Kit Antibody Clone: ACK2
Clone ACK2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD117 / c-Kit Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD117 / c-Kit antibody reveals CD117 / c-Kit expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD117 / c-Kit antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD117 / c-Kit could be seen inepidermal cells in the skin. We were unable to detect CD117 / c-Kit in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD117 / c-Kit expression as measured by anti-CD117 / c-Kit antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
KIT Variability + ++ + + + + + + + + + + + + + + + ++ ++ +

Selected References

Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot - Fischer 344 Rat)Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, D’Addio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, OrCanine T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry - paraffin embedded tissue, in vivo blocking)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD117 / c-Kit Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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