Mouse Anti-CD117 / c-Kit Antibody Product Attributes
Cross Reactivity: rat
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD117 / c-Kit Antibody Clone: ACK2
Clone ACK2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD117 / c-Kit Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Staining with anti-CD117 / c-Kit antibody reveals CD117 / c-Kit expression is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Type:Variations in CD117 / c-Kit antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD117 / c-Kit could be seen inepidermal cells in the skin. We were unable to detect CD117 / c-Kit in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD117 / c-Kit expression as measured by anti-CD117 / c-Kit antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot - Fischer 344 Rat)Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, DÂAddio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, OrCanine T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry - paraffin embedded tissue, in vivo blocking)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD117 / c-Kit Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.