Human Anti-CD20 / MS4A1 Antibody Product Attributes
Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque, capuchin monkey
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD20 / MS4A1 Antibody Clone: 2H7
Clone 2H7 Host and Isotype: Mouse IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD20 / MS4A1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD20 / MS4A1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, germinal center cells in the lymph node, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, germinal center cells in the lymph node, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD20 / MS4A1 could be seen infollicle cells in the ovary and keratinocytes in skin. We were unable to detect CD20 / MS4A1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Selected References
Liu AY, Robinson RR, Murray Jr ED, Ledbetter JA, Hellstrom I and Hellstrom KE. 1987. J Immunol. 139(10): 3521-3526.Schlossman S, Bloumsell L, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.Deans JP, Kalt E, Ledbetter JA, Schieven GL, Bolen JB and Johnson P. 1995. J Biol Chem. 270: 22632-22638. (Flow cytometry)Polyak MJ and Deans JP. 2002. Blood. 99(9): 3256-3262. (Flow cytometry, Immunoprecipitation)Brown KN, Trichel A and Barratt-Boyes SM. 2007. J Immunol. 178(11): 6958-6967. (Flow cytometry - Rhesus)
Limitations and Warranty
enQuire Bio's Human Anti-CD20 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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