Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD20 Antibody [2H7]

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SKU: QAB31
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;APC-Cy7 ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB31-F-25Tests
CD20 / MS4A1 General Information
Alternate Names
CD2, MS4A1, MS4A2, LEU-16, Bp35, B1, CD2, CVID5, S7
Curated Database and Bioinformatic Data
Gene SymbolMS4A1
Entrez Gene ID931
Ensemble Gene IDENSG00000156738
RefSeq Protein Accession(s)NP_068769, NP_690605
RefSeq mRNA Accession(s)NM_152866, NM_021950
RefSeq Genomic Accession(s)NG_023388, NC_018922, NC_000011
UniProt ID(s)P11836, A0A024R507
UniGene ID(s)P11836, A0A024R507
HGNC ID(s)7315
Cosmic ID(s)MS4A1
KEGG Gene ID(s)hsa:931
PharmGKB ID(s)PA31111
General Description of CD20 / MS4A1.
The 2H7 antibody reacts with human CD20, expressed by pre-B cells, resting and activated B cells, follicular dendritic cells and a small subset of T cells, but not on plasma cells. CD20 is a 33-37 kDa transmembrane protein which is differentially phosphorylated in resting and active B cells. The CD20 molecule plays a role in regulating B-cell activation.The 2H7 antibody is reported to be cross-reactive with several non-human species including Baboon, Chimpanzee, Cynomolgus and Rhesus. Please choose the appropriate format for each application.

Human Anti-CD20 / MS4A1 Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque, capuchin monkey
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD20 / MS4A1 Antibody Clone: 2H7
Clone 2H7 Host and Isotype: Mouse IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD20 / MS4A1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD20 / MS4A1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, germinal center cells in the lymph node, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, germinal center cells in the lymph node, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD20 / MS4A1 could be seen infollicle cells in the ovary and keratinocytes in skin. We were unable to detect CD20 / MS4A1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Liu AY, Robinson RR, Murray Jr ED, Ledbetter JA, Hellstrom I and Hellstrom KE. 1987. J Immunol. 139(10): 3521-3526.Schlossman S, Bloumsell L, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.Deans JP, Kalt E, Ledbetter JA, Schieven GL, Bolen JB and Johnson P. 1995. J Biol Chem. 270: 22632-22638. (Flow cytometry)Polyak MJ and Deans JP. 2002. Blood. 99(9): 3256-3262. (Flow cytometry, Immunoprecipitation)Brown KN, Trichel A and Barratt-Boyes SM. 2007. J Immunol. 178(11): 6958-6967. (Flow cytometry - Rhesus)

Limitations and Warranty

enQuire Bio's Human Anti-CD20 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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