Immunohistochemical staining of CD25  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

CD25 [EP218] Antibody (cGMP) – DISC

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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit IgG

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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD229-0.1ml
CD25 General Information
Alternate Names
Molecular Weight
30.8 kDa
Chromosomal Location
p15.1 [chr: 10] [chr_start: 6010689] [chr_end: 6062370] [strand: -1]
Curated Database and Bioinformatic Data
Gene Symbol IL2RA
Entrez Gene ID 3559
RefSeq Protein Accession(s) NP_000408; NP_001295171; NP_001295172
RefSeq mRNA Accession(s) NM_000417; NM_001308242; NM_001308243
RefSeq Genomic Accession(s) NC_000010; NG_007403
UniProt ID(s) P01589
PharmGKB ID(s) PA29828
KEGG Gene ID(s) hsa:3559
Associated Diseases (KEGG IDs) Diabetes mellitus, insulin-dependent, 10 (IDDM10) [MIM:601942]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. {ECO:0000269|PubMed:17676041}. Disease susceptibility is associated with variations affecting the gene represented in this entry.; Immunodeficiency 41 with lymphoproliferation and autoimmunity (IMD41) [MIM:606367]: A disorder of immune dysregulation characterized by recurrent viral, fungal, and bacterial infections, lymphadenopathy, and variable autoimmune features, such as autoimmune enteropathy and eczematous skin lesions. {ECO:0000269|PubMed:23416241, ECO:0000269|PubMed:24116927}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of CD25 .
CD25, or interleukin 2 (IL2) receptor alpha (IL2RA), and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells and activated B cells. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolysis. CD25 also appears to be a reliable immunohistochemical marker for the discrimination of neoplastic from normal/reactive mast cells, with potential as a diagnostic tool in systemic mastocytosis. CD25 is also expressed on hairy cell leukemia


Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at RoomTemperature
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-CD25 Antibody Clone: EP218
Host and Isotype: Rabbit IgG
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody EP218 Staining: Cell Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues of human CD25 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD25 Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD25 [EP218] Antibody (cGMP) - DISC