Human Anti-CD27 / TNFRSF7 Antibody Product Attributes
Cross Reactivity: baboon, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD27 / TNFRSF7 Antibody Clone: O323
Clone O323 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD27 / TNFRSF7 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD27 / TNFRSF7 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD27 / TNFRSF7 could be seen ingerminal center cells in the lymph node and tonsil and hematopoietic cells in the bone marrow. We were unable to detect CD27 / TNFRSF7 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry)Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)Strowig T, Tongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, Eynon EE, Manz, MG, and Flavell RA. 2011. Proc. Natl. Acad. Sci. 108: 13218-13223. (Flow cytometry)So NSY, Ostrowski MA and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (Flow cytometry)Klatt NR, Vinton CL, Lynch RM, Canary LA, Ho J, Darrah PA, Estes JD, Seder RA, Moir SL, and Brenchley JM. 2011. Blood. 118: 5803-5812. (Cell sorting - Rhesus)Ali Z, Yan L, Plagman N, Reichenberg A, Hintz M, Jomaa H, Villinger F, and Chen ZW. 2009. J. Immunol. 183: 5407-5417. (Flow cytometry - Rhesus)Ali Z, Shao L, Halliday L, Reichenberg A, Hintz M, Jomaa H, and Chen ZW. 2007. J. Immunol. 8287-8296. (Flow cytometry - Cynomolgus)
Limitations and Warranty
enQuire Bio's Human Anti-CD27 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.