Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC-Cy7 Mouse IgG1 isotype control.Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug PE Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug V450 Mouse IgG1 isotype control.

Anti-CD27 Antibody [O323]

$ 58.00$ 228.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB36
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;PE ;PE-Cy7 ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB36-F-25Tests
CD27 / TNFRSF7 General Information
Alternate Names
CD27, TNFRSF7, T14, S152. LPFS2, TNFRSF7, S152, Tp55
Curated Database and Bioinformatic Data
Gene SymbolCD27
Entrez Gene ID939
Ensemble Gene IDENSG00000139193
RefSeq Protein Accession(s)XP_011519344, NP_001233, XP_016875722, XP_016875721, XP_016875723
RefSeq mRNA Accession(s)XM_017020232, NM_001242, XM_017020233, XM_011521042, XM_017020234
RefSeq Genomic Accession(s)NC_000012, NG_031995, NC_018923
UniProt ID(s)P26842
UniGene ID(s)P26842
HGNC ID(s)11922
Cosmic ID(s)CD27
KEGG Gene ID(s)hsa:939
PharmGKB ID(s)PA162382107
General Description of CD27 / TNFRSF7.
The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish nave vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.

Human Anti-CD27 / TNFRSF7 Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD27 / TNFRSF7 Antibody Clone: O323
Clone O323 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD27 / TNFRSF7 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD27 / TNFRSF7 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD27 / TNFRSF7 could be seen ingerminal center cells in the lymph node and tonsil and hematopoietic cells in the bone marrow. We were unable to detect CD27 / TNFRSF7 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry)Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)Strowig T, Tongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, Eynon EE, Manz, MG, and Flavell RA. 2011. Proc. Natl. Acad. Sci. 108: 13218-13223. (Flow cytometry)So NSY, Ostrowski MA and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (Flow cytometry)Klatt NR, Vinton CL, Lynch RM, Canary LA, Ho J, Darrah PA, Estes JD, Seder RA, Moir SL, and Brenchley JM. 2011. Blood. 118: 5803-5812. (Cell sorting - Rhesus)Ali Z, Yan L, Plagman N, Reichenberg A, Hintz M, Jomaa H, Villinger F, and Chen ZW. 2009. J. Immunol. 183: 5407-5417. (Flow cytometry - Rhesus)Ali Z, Shao L, Halliday L, Reichenberg A, Hintz M, Jomaa H, and Chen ZW. 2007. J. Immunol. 8287-8296. (Flow cytometry - Cynomolgus)

Limitations and Warranty

enQuire Bio's Human Anti-CD27 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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