Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Monoclonal Antibody (135-4C5).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Monoclonal Antibody (135-4C5).

Anti-CD45 / LCA Antibody Clone 135-4C5

(1 customer review)
$ 129.00$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5788-MSM9
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5788-MSM9-P0 Size: 20 ug, Tag: Unconjugated $ 129.00
5788-MSM9-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 5788-MSM9-P1
CD45 / LCA General Information
Alternate Names
Protein tyrosine phosphatase, receptor type, C, PTPRC
Molecular Weight
180-220kDa
Chromosomal Location
1q31.3
Curated Database and Bioinformatic Data
Gene SymbolPTPRC
Entrez Gene ID5788
Ensemble Gene IDENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)9666
Cosmic ID(s)PTPRC
KEGG Gene ID(s)hsa:5788
PharmGKB ID(s)PA34011
General Description of CD45 / LCA.
CD45R, also designated CD45, PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5,, 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6), CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. This MAb reacts with all isoforms of CD45R expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes (Workshop IV). Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Human Anti-CD45 / LCA Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD45 / LCA Antibody Clone: 135-4C5
Clone 135-4C5 Host and Isotype: Mouse IgG2b kappa
Anti-Human CD45 / LCA Positive Control Sample: Ramos, U-698, or GA-10 cells. Tonsil
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Stimulated human leukocytes
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD45 / LCA Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45 / LCA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45 / LCA could be seen in. We were unable to detect CD45 / LCA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45 / LCA expression as measured by anti-CD45 / LCA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's CD45 / LCA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
  1. I have tried a ton of CD45 primary antibodies and finally decided this one. For me, it recognizes CD45 on leucocytes in the bloodstream. The picture shows a spike experiment (cancer cell into healthy blood), CK in Cy3, CD45 shows up in green. I have two other isotypes in the panel for multi-color staining: mouse IgG2a and mouse IgG1. They do not cross-react if use the correct secondary.

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Anti-CD45 / LCA Antibody Clone 135-4C5

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