Formalin-fixed, paraffin-embedded human Brain Tumor stained with CD56 Monoclonal Antibody (123A8)
Formalin-fixed, paraffin-embedded human Brain Tumor stained with CD56 Monoclonal Antibody (123A8)

Anti-CD56 / NCAM1 / NKH1 Antibody Clone 123A8; same 56C04

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4684-MSM2
Species: Human
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4684-MSM2-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 4684-MSM2-P1
CD56 / NCAM1 / NKH1 General Information
Alternate Names
Neural cell adhesion molecule, NCAM, CD56
Molecular Weight
180, 145, 125kDa
Chromosomal Location
11q23.1
Curated Database and Bioinformatic Data
Gene SymbolNCAM1
Entrez Gene ID4684
Ensemble Gene IDENSG00000149294
RefSeq Protein Accession(s)NP_001229536, NP_851996, NP_001070150, NP_001229537, NP_000606
RefSeq mRNA Accession(s)NM_001076682, NM_001242607, NM_001242608, NM_181351 NM_000615
RefSeq Genomic Accession(s)NC_000011, NG_032036, NC_018922
UniProt ID(s)P13591, A0A087WWD4
UniGene ID(s)P13591, A0A087WWD4
HGNC ID(s)7656
Cosmic ID(s)NCAM1
KEGG Gene ID(s)hsa:4684
PharmGKB ID(s)PA31459
General Description of CD56 / NCAM1 / NKH1.
This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues, neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas,, small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal, nasal NK/T-cell lymphomas.

Human Anti-CD56 / NCAM1 / NKH1 Antibody Product Attributes

Species: Human
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD56 / NCAM1 / NKH1 Antibody Clone: 123A8; same 56C04
Clone 123A8; same 56C04 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD56 / NCAM1 / NKH1 Positive Control Sample: Cerebellum, Pancreas, Neuroblastoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Membrane preparation of a small cell lung carcinoma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD56 / NCAM1 / NKH1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NCAM1 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD56 / NCAM1 / NKH1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. Low, but measureable presence of CD56 / NCAM1 / NKH1 could be seen inglial cells in the hippocampus, exocrine glandular cells in the pancreas and smooth muscle cells in the smooth muscle. We were unable to detect CD56 / NCAM1 / NKH1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD56 / NCAM1 / NKH1 expression as measured by anti-CD56 / NCAM1 / NKH1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - ++ - - - - - - - - - - - - - -
NCAM1 Variability + ++ + + + ++ + + + + + ++ + + + + + + ++ +

Limitations and Warranty

enQuire Bio's CD56 / NCAM1 / NKH1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-CD56 / NCAM1 / NKH1 Antibody Clone 123A8; same 56C04”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-CD56 / NCAM1 / NKH1 Antibody Clone 123A8; same 56C04

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-4684-MSM2-P1-anti-CD56-NCAM1-NKH1-antibody-300x225.jpeg