C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3e  and 0.125 ug APC Mouse Anti-CD90.2 (QAB52) (right panel) or 0.125 ug APC Rat IgG2b isotype control (left panel).
C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3e  and 0.125 ug APC Mouse Anti-CD90.2 (QAB52) (right panel) or 0.125 ug APC Rat IgG2b isotype control (left panel).C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Mouse Anti-CD90.2 .C57Bl/6 splenocytes were stained with 0.125 ug PE Mouse Anti-CD90.2 (QAB52) (solid line) or 0.125 ug PE Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Mouse Anti-CD90.2 .C57Bl/6 splenocytes were stained with 0.125 ug V450 Mouse Anti-CD90.2  (solid line) or 0.125 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3e  and 0.125 ug APC Mouse Anti-CD90.2 (QAB52) (right panel) or 0.125 ug APC Rat IgG2b isotype control (left panel).C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Mouse Anti-CD90.2 .C57Bl/6 splenocytes were stained with 0.125 ug PE Mouse Anti-CD90.2 (QAB52) (solid line) or 0.125 ug PE Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Mouse Anti-CD90.2 .C57Bl/6 splenocytes were stained with 0.125 ug V450 Mouse Anti-CD90.2  (solid line) or 0.125 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD90.2 Antibody [30-H12]

$ 48.00$ 198.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB52
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;V450
Isotype: Rat IgG2b
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB52-F-25ug
CD90.2 General Information
Alternate Names
Thy-1.2
Curated Database and Bioinformatic Data
Gene SymbolThy1
Entrez Gene ID21838
Ensemble Gene IDENSMUSG00000032011
RefSeq Protein Accession(s)NP_033408
RefSeq mRNA Accession(s)NM_009382
RefSeq Genomic Accession(s)NC_000075
UniProt ID(s)P01831
UniGene ID(s)P01831
Cosmic ID(s)Thy1
KEGG Gene ID(s)mmu:21838
General Description of CD90.2.
The 30-H12 antibody reacts with mouse CD90.2 (Thy1.2). CD90.2 is a strain-specific allelic form of the GPI-linked membrane associated protein CD90 and is involved in adhesion and signal transduction. CD90.2 is expressed on thymocytes, mature T cells and neurons in mouse strains that express the CD90.2 allele (BALB/c, CBA, C3H, C57BL/6, SJL and others). 30-H12 does not react with the CD90.1 allele expressed in mouse strains such as PL and AKR.

Mouse Anti-CD90.2 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD90.2 Antibody Clone: 30-H12
Clone 30-H12 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD90.2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD90.2 antibody reveals CD90.2 expression is expected to be primarily localized to the nucleus and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD90.2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD90.2 could be seen inperipheral nerve/ganglion in colon, cells in the tubules in kidney and smooth muscle cells in the smooth muscle. We were unable to detect CD90.2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Ledbetter JA and Herzenberg LA. 1979. Immunol. Rev. 47:63-90. Borrello MA and Phipps RP. 1996. Cell Immunol. 173:198-206. (flow cytometry) Seaman WE, Wofsy D, Greenspan JS, and Ledbetter JA. 1983. J. Immunol. 130: 1713-8. (depletion)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD90.2 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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