Immunohistochemical staining of Cdc25A  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cdc25A [DCS-120] Antibody (cGMP).

$ 158.00$ 898.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD261
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a

Product NumberDescriptionPrice
QIVD261-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD261-0.5ml Size: 0.5 ml, Format: Concentrate $ 475.00
QIVD261-1ml Size: 1 ml, Format: Concentrate $ 795.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD261-0.1ml
Cdc25A General Information
Alternate Names
Molecular Weight
59.1 kDa
Chromosomal Location
p21.31 [chr: 3] [chr_start: 48157146] [chr_end: 48188402] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolCDC25A
Entrez Gene ID993
RefSeq Protein Accession(s)NP_963861; NP_001780; XP_011532618
RefSeq mRNA Accession(s)XM_006713434; XR_001740392; NM_201567; NM_001789; XM_006713435
RefSeq Genomic Accession(s)NC_000003
UniProt ID(s)P30304
PharmGKB ID(s)PA26259
KEGG Gene ID(s)hsa:993
General Description of Cdc25A .
This antibody reacts with a 65 kDa protein known as Cdc25A. The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1-S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cdc25A Antibody Clone: DCS-120
Host and Isotype: Mouse IgG2a
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody DCS-120 Staining: Nuclear membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with purified recombinant Cdc25A.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cdc25A Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Cdc25A [DCS-120] Antibody (cGMP).