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Anti-Chromogranin A / CHGA Antibody Clone CGA/414

$ 298.00
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SKU: 1113-MSM1
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1113-MSM1-P1
Chromogranin A / CHGA General Information
Alternate Names
TIMP metallopeptidase inhibitor 1, TIMP1, Tissue Inhibitor of Metalloproteinase 1
Molecular Weight
68-75kDa
Chromosomal Location
14q32.12
Curated Database and Bioinformatic Data
Gene SymbolCHGA
Entrez Gene ID1113
Ensemble Gene IDENSG00000276781, ENSG00000100604
RefSeq Protein Accession(s)NP_001288619, XP_011534672, NP_001266
RefSeq mRNA Accession(s)XM_011536370, NM_001301690, NM_001275
RefSeq Genomic Accession(s)NC_000014, NG_187601, NC_018925
UniProt ID(s)Q86T07, P10645, G5E968
UniGene ID(s)Q86T07, P10645, G5E968
HGNC ID(s)1929
Cosmic ID(s)CHGA
KEGG Gene ID(s)hsa:1113
PharmGKB ID(s)PA26461
General Description of Chromogranin A / CHGA.
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large, small intestine, adrenal medulla, pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas,, other neuroendocrine tumors. Co-expression of chromogranin A, neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins, chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining, absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin, NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas, prolactinomas readily express chromogranin.

Human and Rat (-) Anti-Chromogranin A / CHGA Antibody Product Attributes

Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Chromogranin A / CHGA Antibody Clone: CGA/414
Clone CGA/414 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat (-) Chromogranin A / CHGA Positive Control Sample: PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.
Cellular Localization of Antibody Finely granular cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human chromogranin A protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Chromogranin A / CHGA Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CHGA antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Chromogranin A / CHGA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the parathyroid gland and islets of Langerhans in pancreas. More moderate antibody staining intensity was present in glandular cells in the parathyroid gland and islets of Langerhans in pancreas. Low, but measureable presence of Chromogranin A / CHGA could be seen inneuronal cells in the caudate nucleus, cerebral cortex and hippocampus, respiratory epithelial cells in the bronchus and neuronal cells in the caudate nucleus and cerebral cortex and hippocampus. We were unable to detect Chromogranin A / CHGA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Chromogranin A / CHGA expression as measured by anti-Chromogranin A / CHGA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - +++ - - - - - - - - - - - - - - - - - -
CHGA Variability + + + + ++ + ++ + + + + + + + + ++ ++ + + +

Limitations and Warranty

enQuire Bio's Chromogranin A / CHGA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Chromogranin A / CHGA Antibody Clone CGA/414