Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:50-1:100|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Cyclooxygenase-2 Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Lung and colon carcinoma
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic, membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant protein corresponding to C-terminus of rat COX2.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Cyclooxygenase-2 Information for Pathologists
Summary:Not related to Cytochrome c oxidase (COX). Cyclooxygenases 1 (COX1) and 2 (COX2), also known as prostaglandin H synthase, catalyze formation of prostaglandin from arachidonic acid. COX1 is constitutive form of enzyme on 9q; COX2 is inducible isoform on 1q, has 61% sequence homology with COX1. Regulated by mitogens, tumor promoters, cytokines, serum, free fatty acids, NSAIDs, selective COX2 inhibitors. Increased expression associated with poor clinical outcome in stage I/II non-small cell lung carcinoma (Arch Pathol Lab Med 2005;129:1113), possibly poorer outcome in follicular thyroid carcinoma (Arch Pathol Lab Med 2005;129:736).
Common Uses By Pathologists:Endothelial cells (cytoplasmic) as well as breast, colorectal, esophageal, liver, lung, ovary, pancreas, prostate, skin, stomach tumors stain positive for COX2.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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