Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CK17 Monoclonal Antibody (KRT17/778).
Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CK17 Monoclonal Antibody (KRT17/778).

Anti-Cytokeratin 17 Antibody Clone KRT17/778

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3872-MSM2
Species: Human, Rat, Cow, Goat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3872-MSM2-P1
Cytokeratin 17 General Information
Alternate Names
Keratin, type I cytoskeletal 17, cytokeratin 17, KRT17
Molecular Weight
46kDa
Chromosomal Location
17q21.2
Curated Database and Bioinformatic Data
Gene SymbolKRT17
Entrez Gene ID3872
Ensemble Gene IDENSG00000128422
RefSeq Protein Accession(s)NP_000413
RefSeq mRNA Accession(s)NM_000422
RefSeq Genomic Accession(s)NC_018928, NG_008625, NG_009090, NC_000017
UniProt ID(s)Q14666, Q04695
UniGene ID(s)Q14666, Q04695
HGNC ID(s)6427
Cosmic ID(s)KRT17
KEGG Gene ID(s)hsa:3872
PharmGKB ID(s)PA30214
General Description of Cytokeratin 17.
Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat, salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells, skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17, anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2, anti-CDX-2 positivity defines intestinal subtype.

Human, Rat, Cow, Goat, and Pig Anti-Cytokeratin 17 Antibody Product Attributes

Species: Human, Rat, Cow, Goat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 17 Antibody Clone: KRT17/778
Clone KRT17/778 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Rat, Cow, Goat, and Pig Cytokeratin 17 Positive Control Sample: T24 cells or Skin. Bladder or Cervix.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human KRT17 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 17 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-krt17 antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 17 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the prostate and salivary gland, myoepithelial cells in the breast and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in glandular cells in the prostate and salivary gland, myoepithelial cells in the breast and urothelial cells in the urinary bladder. Low, but measureable presence of Cytokeratin 17 could be seen inexocrine glandular cells in the pancreas, glandular cells in the fallopian tube and squamous epithelial cells in the esophagus and oral mucosa. We were unable to detect Cytokeratin 17 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 17 expression as measured by anti-Cytokeratin 17 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - +++ - - - +++ - ++ - - - - - - +++ - - - +++
KRT17 Variability + + + + ++ + ++ ++ ++ + + ++ ++ + + ++ + + + ++

Limitations and Warranty

enQuire Bio's Cytokeratin 17 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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