Formalin-fixed, paraffin-embedded human Skin stained with CK17 Monoclonal Antibody (SPM56).
Formalin-fixed, paraffin-embedded human Skin stained with CK17 Monoclonal Antibody (SPM56).Western Blot of HeLa Lysate using CK17 Monoclonal Antibody (SPM56).

Anti-Cytokeratin 17 Antibody Clone SPM560

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3872-MSM1X
Species: Human, Rat, Cow, Goat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
3872-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: 3872-MSM1X-P1
Cytokeratin 17 General Information
Alternate Names
Keratin, type I cytoskeletal 17, cytokeratin 17, KRT17
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolKRT17
Entrez Gene ID3872
Ensemble Gene IDENSG00000128422
RefSeq Protein Accession(s)NP_000413
RefSeq mRNA Accession(s)NM_000422
RefSeq Genomic Accession(s)NC_018928, NG_008625, NG_009090, NC_000017
UniProt ID(s)Q14666, Q04695
UniGene ID(s)Q14666, Q04695
HGNC ID(s)6427
Cosmic ID(s)KRT17
KEGG Gene ID(s)hsa:3872
PharmGKB ID(s)PA30214
General Description of Cytokeratin 17.
Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands, other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat, salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells, skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5&6, p63,, SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC), lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR, Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17, anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2, anti-CDX-2 positivity defines intestinal subtype.

Human, Rat, Cow, Goat, and Pig Anti-Cytokeratin 17 Antibody Product Attributes

Species: Human, Rat, Cow, Goat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Frozen & formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 17 Antibody Clone: SPM560
Clone SPM560 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Rat, Cow, Goat, and Pig Cytokeratin 17 Positive Control Sample: Skin
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: The cytoskeletal fraction of rat colon epithelium
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 17 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-krt17 antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 17 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the prostate and salivary gland, myoepithelial cells in the breast and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in glandular cells in the prostate and salivary gland, myoepithelial cells in the breast and urothelial cells in the urinary bladder. Low, but measureable presence of Cytokeratin 17 could be seen inexocrine glandular cells in the pancreas, glandular cells in the fallopian tube and squamous epithelial cells in the esophagus and oral mucosa. We were unable to detect Cytokeratin 17 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 17 expression as measured by anti-Cytokeratin 17 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - +++ - - - +++ - ++ - - - - - - +++ - - - +++
KRT17 Variability + + + + ++ + ++ ++ ++ + + ++ ++ + + ++ + + + ++

Limitations and Warranty

enQuire Bio's Cytokeratin 17 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-Cytokeratin 17 Antibody Clone SPM560”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title




  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-Cytokeratin 17 Antibody Clone SPM560