Anti-Cytokeratin 19Antibody Validation Data
Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 19 MS-QAVA™ monoclonal antibody [Clone: A53-B/A2.26] (solid-line), reveals that less than 11% of signal is attributable to non-specific binding of anti-Cytokeratin 19 [Clone A53-B/A2.26] to targets other than KRT19 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.

Anti-Cytokeratin 19 Antibody Clone A53-B/A2.26

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3880-MSM1
Species: Human, Rat (-), and Ferret (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: MS-QAVA™ Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3880-MSM1-P1
Cytokeratin 19 General Information
Alternate Names
Keratin, type I cytoskeletal 19, cytokeratin-19, CK-19, keratin-19, K19, KRT19
Molecular Weight
40kDa
Chromosomal Location
17q21.2
Curated Database and Bioinformatic Data
Gene SymbolKRT19
Entrez Gene ID3880
Ensemble Gene IDENSG00000171345
RefSeq Protein Accession(s)NP_002267
RefSeq mRNA Accession(s)NM_002276
RefSeq Genomic Accession(s)NC_000017, NC_018928, NG_012285
UniProt ID(s)P08727
UniGene ID(s)P08727
HGNC ID(s)6436
Cosmic ID(s)KRT19
KEGG Gene ID(s)hsa:3880
PharmGKB ID(s)PA30225
General Description of Cytokeratin 19.
This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal, secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus,, ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver,, breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow, breast cancer.

Human, Rat (-), and Ferret (-) Anti-Cytokeratin 19 Antibody Product Attributes

Species: Human, Rat (-), and Ferret (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Cytokeratin 19 Antibody Clone: A53-B/A2.26
Clone A53-B/A2.26 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Rat (-), and Ferret (-) Cytokeratin 19 Positive Control Sample: MCF-7, HeLa, Hep-G2 cells, Breast Cancer
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human breast cancer MCF-7 cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 19 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Cytokeratin 19 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the tonsil, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the tonsil, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Cytokeratin 19 could be seen inepidermal cells in the skin, squamous epithelial cells in the cervix and uterine and oral mucosa. We were unable to detect Cytokeratin 19 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 19 expression as measured by anti-Cytokeratin 19 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ +++ +++ +++ +++ - + - +++ - - +++ +++ +++ + ++ +++ - +++ +++
KRT19 Variability + + + + + + ++ ++ + + + + + ++ +++ ++ + + ++ +

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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