![Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 8 Anti-Human MS-QAVA™ monoclonal antibody [Clone: K8/383] (solid-line), reveals that less than 13% of signal is attributable to non-specific binding of anti-Cytokeratin 8 Anti-Human [Clone: K8/383 ] to targets other than KRT8 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-3856-MSM3-P1-anti-Cytokeratin-8-Anti-Human-antibody.png)
Human Anti-Cytokeratin 8 Anti-Human Antibody Product Attributes
Species: Human
Tested Applications: Western Blot. Additionally, this antibody has been validated by MS-QAVAâ¢. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Cytokeratin 8 Anti-Human Antibody Clone: K8/383
Clone K8/383 Host and Isotype: Mouse IgG1
Anti-Human Cytokeratin 8 Anti-Human Positive Control Sample: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma
Cellular Localization of Antibody <K8/383 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length cytokeratin 8 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 8 Anti-Human Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Variations in Cytokeratin 8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the appendix, breast, cervix, uterine, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, seminal vesicle, small intestine, stomach and thyroid gland, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in bile duct cells in the liver, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the appendix, breast, cervix, uterine, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, seminal vesicle, small intestine, stomach and thyroid gland, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Cytokeratin 8 could be seen in. We were unable to detect Cytokeratin 8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Type:
Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 8 expression as measured by anti-Cytokeratin 8 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | +++ | +++ | +++ | +++ | +++ | - | - | +++ | ++ | - | - | +++ | +++ | +++ | ++ | - | +++ | + | +++ | +++ |
| KRT8 Variability | + | + | ++ | + | ++ | + | ++ | + | ++ | + | + | ++ | + | + | ++ | ++ | + | ++ | + | ++ |
Limitations and Warranty
This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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