Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-EpCAM Antibody Clone: E29
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Breast carcinoma
Cellular Localization of Antibody E29 Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with delipidated human milk fat globule membrane preparation.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
EpCAM Information for Pathologists
Summary:
Antibody to cell membrane glycoproteins expressed on healthy epithelia and in various carcinomas. Also known as epithelial cell adhesion molecule, BerEp4 (Ber-EP4, J Clin Pathol 1990;43:213), MOC31 (MOC-31, Acta Neuropathol 1991;83:46), CD326, TACSTD1 protein. MOC31 and BerEP4 are both anti-EpCAM antibodies, MOC31 appears to be superior (Appl Immunohistochem Mol Morphol 2009;17:202). Anti-EpCam antibodies are in clinical trials for patients with cancer (Br J Cancer 2007;96:417). Uses by pathologistsCommon Uses By Pathologists:
Membranous staining. Sensitive and specific for lung adenocarcinoma (positive) vs. mesothelioma (negative, Am J Surg Pathol 2001;25:43). May help distinguish, as part of a panel, hepatocellular carcinoma (usually negative) from metastatic adenocarcinoma to liver or cholangiocarcinoma (usually positive, Mod Pathol 2002;15:1279). Immunoexpression may predict poor survival in carcinomas of breast, gallbladder (Am J Clin Pathol 2008;129:424), ovary, pancreas. Microscopic (histologic) imagesLimitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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