Immunohistochemical staining of Epithelial Antigen (EpCAM)  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Epithelial Antigen (EpCAM) [EP155] Antibody (cGMP).

$ 135.00$ 745.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD135
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Rabbit IgG

Product NumberDescriptionPrice
QIVD135-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD135-0.5ml Size: 0.5 ml, Format: Concentrate $ 465.00
QIVD135-1ml Size: 1 ml, Format: Concentrate $ 745.00
QIVD135-6ml Size: 6 ml, Format: Predilute $ 355.00
QIVD135-10ml Size: 10 ml, Format: Predilute $ 595.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QIVD135-0.1ml
Epithelial Antigen (EpCAM) General Information
Alternate Names
Molecular Weight
34.9 kDa
Chromosomal Location
p21 [chr: 2] [chr_start: 47345158] [chr_end: 47387601] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolEPCAM
Entrez Gene ID4072
RefSeq Protein Accession(s)NP_002345
RefSeq mRNA Accession(s)NM_002354;
RefSeq Genomic Accession(s)NG_012352; NC_000002
UniProt ID(s)P16422
PharmGKB ID(s)PA35493
KEGG Gene ID(s)hsa:4072
Associated Diseases (KEGG IDs)Diarrhea 5, with tufting enteropathy, congenital (DIAR5) [MIM:613217]: An intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum. {ECO:0000269|PubMed:18572020}. The disease is caused by mutations affecting the gene represented in this entry.; Hereditary non-polyposis colorectal cancer 8 (HNPCC8) [MIM:613244]: An autosomal dominant disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. {ECO:0000269|PubMed:19098912}. The disease is caused by mutations affecting the gene represented in this entry. HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
General Description of Epithelial Antigen (EpCAM) .
Ep-CAM is a highly conserved typeI transmembrane glycoprotein and is expressed on most normal and malignant epithelial cells. Ep-CAM is also known as epithelial cell adhesion molecule. It is detected at the membrane/cytoplasm of epithelial tissues. Ep-CAM is over expressed by the majority of human epithelial carcinomas except hepatocellular carcinoma. An antibody to EP-CAM is useful in identification of epithelial tumors and to differentiate primary hepatocellular carcinoma from liver metastasis

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:100 - 1:200
Pretreatment EDTA Buffer pH8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Epithelial Antigen (EpCAM) Antibody Clone: EP155
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Colon for normal tissue and colorectal cancer for tumor tissue
Cellular Localization of Antibody EP155 Staining: Membrane/Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to amino acid residues in human Epithelial Antigen (Ep-CAM) protein was used as an immunogen
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Epithelial Antigen (EpCAM) Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

There are no reviews yet.

Be the first to review “Epithelial Antigen (EpCAM) [EP155] Antibody (cGMP).”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Epithelial Antigen (EpCAM) [EP155] Antibody (cGMP).