Western Blot of Estrogen Receptor in MCF-7 Lysate using Estrogen Receptor Ab (SPM567).
Western Blot of Estrogen Receptor in MCF-7 Lysate using Estrogen Receptor Ab (SPM567).

Anti-Estrogen Receptor Antibody Clone SPM567

$ 298.00
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SKU: 2099-MSM1X
Species: Human
Tested Applications: Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2099-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock: 1-2 Day Lead Time.
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Datasheets and Documentation
Product Datasheet
SKU: 2099-MSM1X-P1
Estrogen Receptor General Information
Alternate Names
Estrogen receptor alpha, ER-?, ERalpha, ER alpha, ESR1
Molecular Weight
~67kDa
Chromosomal Location
6q25.1
Curated Database and Bioinformatic Data
Gene SymbolESR1
Entrez Gene ID2099
Ensemble Gene IDENSG00000091831
RefSeq Protein Accession(s)XP_011533846, XP_016865866, XP_016865871, NP_001315029, XP_011533845, XP_016865867, XP_016865870, XP_016865872, XP_006715438, XP_016865865, XP_016865869, NP_000116, NP_001116212, XP_011533849, NP_001116213, XP_011533847, XP_011533851, XP_016865868, XP_006715437, NP_001116214, NP_001278159, NP_001278170
RefSeq mRNA Accession(s)XM_011535547, NM_000125, XM_011535544, XM_017010376 NM_001122742, XM_017010380, XM_017010381, XM_011535543, XM_017010378, XR_001743222, NM_001328100, XM_011535549, XM_017010377, XM_017010379, XM_017010382, NM_001122740, NM_001122741, NM_001291241, XM_011535545, XM_017010383, XM_006715374, XM_006715375, XR_001743223, NM_001291230
RefSeq Genomic Accession(s)NC_000006, NC_018917, NG_008493
UniProt ID(s)A8KAF4, A0A125SXW3, Q9UBT1, H0Y4W6, P03372, G4XH65
UniGene ID(s)A8KAF4, A0A125SXW3, Q9UBT1, H0Y4W6, P03372, G4XH65
HGNC ID(s)3467
Cosmic ID(s)ESR1
KEGG Gene ID(s)hsa:2099
PharmGKB ID(s)PA156
General Description of Estrogen Receptor.
This MAb is specific to ER alpha, shows minimal cross-reaction with other members of the family. ER is an important regulator of growth, differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This MAb is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.

Human Anti-Estrogen Receptor Antibody Product Attributes

Species: Human
Tested Applications: Western Blot, Immunohistochemistry (IHC).
Application Notes: Western Blot (0.5-1ug of antibody/ml for 2 hours at RT), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Estrogen Receptor Antibody Clone: SPM567
Clone SPM567 Host and Isotype: Mouse IgG1 kappa
Anti-Human Estrogen Receptor Positive Control Sample: MCF-7 Cells or Breast Cancers.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Estrogen Receptor alpha protein (aa2-185)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Estrogen Receptor Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ESR1 antibody staining is expected to be primarily localized to the nucleus and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Estrogen Receptor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, glandular cells in the cervix, uterine, endometrium and fallopian tube, squamous epithelial cells in the cervix and uterine. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, glandular cells in the cervix, uterine, endometrium and fallopian tube, squamous epithelial cells in the cervix and uterine. Low, but measureable presence of Estrogen Receptor could be seen insmooth muscle cells in the smooth muscle. We were unable to detect Estrogen Receptor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Estrogen Receptor expression as measured by anti-Estrogen Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ - - - + - - - - - - - - - - - - - - -
ESR1 Variability ++ ++ + + ++ + + + + + + ++ + + + + + + + +

Limitations and Warranty

enQuire Bio's Estrogen Receptor Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Estrogen Receptor Antibody Clone SPM567

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