.jpg "Galectin-1 IHC Human Prostate Carcinoma (GAL1 1831)")
Human Anti-Galectin-1 Antibody Product Attributes
Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Galectin-1 Antibody Clone: GAL1/1831
Clone GAL1/1831 Host and Isotype: Mouse IgG1 lambda
Anti-Human Galectin-1 Positive Control Sample: HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.
Cellular Localization of Antibody Secreted (extracellular matrix)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Galectin-1 protein fragment (aa12-108)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Galectin-1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-LGALS1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm. There is variability in either the signal strength or the localization of signal in cytosol, nucleoplasm and cytosol from cell to cell.Observed Antibody Staining Data By Tissue Type:
Variations in Galectin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in adipocytes in mesenchymal tissue, cells in the endometrial stroma in endometrium, decidual cells in the placenta and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in adipocytes in mesenchymal tissue, cells in the endometrial stroma in endometrium, decidual cells in the placenta and smooth muscle cells in the smooth muscle. Low, but measureable presence of Galectin-1 could be seen in cells in the glomeruli in kidney, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, cells in the white pulp in spleen, chondrocytes in mesenchymal tissue, fibroblasts in skin and mesenchymal tissue, germinal center cells in the lymph node, glandular cells in the parathyroid gland, glial cells in the caudate nucleus and hippocampus, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, macrophages in lung, neuronal cells in the cerebral cortex and hippocampus, neuropil in cerebral cortex, non-germinal center cells in the lymph node and tonsil, pneumocytes in lung, squamous epithelial cells in the cervix and uterine and vagina. We were unable to detect Galectin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Galectin-1 expression as measured by anti-Galectin-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | - | - | + | ++ | ++ | - | - | - | +++ | - | - | - | - | - | - | - | + | - |
| LGALS1 Variability | ++ | ++ | + | + | +++ | +++ | ++ | + | ++ | + | ++ | + | + | + | ++ | ++ | + | + | ++ | ++ |
Limitations and Warranty
enQuire Bio's Galectin-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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