Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Glycophorin A Monoclonal Antibody (GYPA/28)

Anti-Glycophorin A / CD235a Antibody Clone GYPA/280

Name: Anti-Glycophorin A / CD235a Antibody Clone GYPA/280
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 2993-MSM4
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
2993-MSM4-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

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SKU: 2993-MSM4-P1

Glycophorin A / CD235a General Information
Alternate Names
Glycophorin A, MNS blood group, GYPA
Molecular Weight
39kDa
Chromosomal Location
4q31.22
Curated Database and Bioinformatic Data
Gene Symbol	GYPA
Entrez Gene ID	2993, 2994
Ensemble Gene ID	ENSG00000170180, ENSG00000250361
RefSeq Protein Accession(s)	XP_016863624, NP_001295116, XP_016863623, NP_001295119, XP_016863625, NP_002090, NP_001291311, XP_011530207, XP_011530206, XP_016863626, XP_011530205, NP_002091
RefSeq mRNA Accession(s)	XM_017008134, XM_017008135, NM_001308187 NM_001308190, NM_002099, XM_017008136, XM_011531903, NM_002100, XM_011531904, NM_001304382, XM_011531905, XM_017008137
RefSeq Genomic Accession(s)	NC_018915, NG_007470, NC_000004, NC_000004, NC_018915, NG_007483
UniProt ID(s)	E9PD10, A0A0C4DFT7, Q14419, P02724, P06028, B8Q175
UniGene ID(s)	E9PD10, A0A0C4DFT7, Q14419, P02724, P06028, B8Q175
HGNC ID(s)	4702, 4703
Cosmic ID(s)	GYPA, GYPB
KEGG Gene ID(s)	hsa:2993, hsa:2994
PharmGKB ID(s)	PA29080, PA29081
General Description of Glycophorin A / CD235a.
Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC), erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus, parvovirus. Glycophorins A (GPA), B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M, N specificities, while GPB accounts for S, U specificities. GPA, GPB provide the cells with a large mucin like surface, it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Human Anti-Glycophorin A / CD235a Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-Glycophorin A / CD235a Antibody Clone: GYPA/280

Clone GYPA/280 Host and Isotype: Mouse IgG1 kappa

Anti-Human Glycophorin A / CD235a Positive Control Sample: Erythrocytes in any human tissues

Cellular Localization of Antibody Cell surface

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant human glycophorin A protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Glycophorin A / CD235a Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Glycophorin A / CD235a antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Glycophorin A / CD235a could be seen in. We were unable to detect Glycophorin A / CD235a in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Glycophorin A / CD235a Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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