Formalin-fixed, paraffin-embedded human Tonsil stained with MHC I Monoclonal Antibody (SPM418).
Formalin-fixed, paraffin-embedded human Tonsil stained with MHC I Monoclonal Antibody (SPM418).

Anti-HLA-Aw32 & HLA-A25 Antibody Clone SPM418

$ 298.00
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SKU: 3105-MSM4X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3105-MSM4X-P1
HLA-Aw32 & HLA-A25 General Information
Alternate Names
HLA-A, HLA class A, MHC I
Chromosomal Location
6q21.3
Curated Database and Bioinformatic Data
Gene SymbolHLA-A
Entrez Gene ID3105
Ensemble Gene IDENSG00000229215, ENSG00000231834, ENSG00000224320, ENSG00000235657, ENSG00000206503, ENSG00000227715, ENSG00000206505, ENSG00000223980
RefSeq Protein Accession(s)NP_002107, XP_005275388, NP_001229687, XP_016885777
RefSeq mRNA Accession(s)XR_430999 NM_002116, XM_005275331, XM_017030288, NM_001242758
RefSeq Genomic Accession(s)NG_167247, NG_167248, NG_167249, NG_029217, NG_167245, NC_018917, NG_167246, NC_000006, NG_113891, NG_167244
UniProt ID(s)O78126, P30443, P04439, B1PKY1, B2R7U3, Q5SUL5
UniGene ID(s)O78126, P30443, P04439, B1PKY1, B2R7U3, Q5SUL5
HGNC ID(s)4931
Cosmic ID(s)HLA-A
KEGG Gene ID(s)hsa:3105
PharmGKB ID(s)PA35055
General Description of HLA-Aw32 & HLA-A25.
This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B, HLA-C, belongs to major histocompatibility complex (MHC) class I antigens, expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity,, rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25, HLA-Aw32.

Human Anti-HLA-Aw32 & HLA-A25 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-HLA-Aw32 & HLA-A25 Antibody Clone: SPM418
Clone SPM418 Host and Isotype: Mouse IgG2a kappa
Anti-Human HLA-Aw32 & HLA-A25 Positive Control Sample: THP-1, Jurkat cells. Tonsil or lymph node.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

HLA-Aw32 & HLA-A25 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-hla-a antibody staining is expected to be primarily localized to the plasma membrane and golgi apparatus.

Limitations and Warranty

enQuire Bio's HLA-Aw32 & HLA-A25 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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