C57Bl/6 splenocytes were stimulated with PMA and Ionomycin , followed by intracellular staining with 0.06 ug APC Mouse Anti-IFN gamma (QAB83).

Mouse Anti-IFN gamma Antibody [XMG1.2]

$ 49.00$ 269.00
Please Select A Product To View The SKU.

SKU: QAB83
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;APC ;FITC ;PE ;PE-Cy7 ;V450
Isotype: Rat IgG1 kappa
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QAB83-B-25ug
IFNG / Interferon Gamma General Information
Alternate Names
IFNG, Interferon Gamma Protein, EGK_391, IFN-gamma
Curated Database and Bioinformatic Data
Gene SymbolIfng
Entrez Gene ID15978
Ensemble Gene IDENSMUSG00000055170
RefSeq Protein Accession(s)NP_032363
RefSeq mRNA Accession(s)NM_008337
RefSeq Genomic Accession(s)NC_000076
UniProt ID(s)P01580
UniGene ID(s)P01580
Cosmic ID(s)Ifng
KEGG Gene ID(s)mmu:15978
General Description of IFNG / Interferon Gamma.
The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of IFN-g in a variety of assays (use format suitable for functional assays).

Mouse Anti-IFNG / Interferon Gamma Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-IFNG / Interferon Gamma Antibody Clone: XMG1.2
Clone XMG1.2 Host and Isotype: Rat IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

IFNG / Interferon Gamma Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in IFNG / Interferon Gamma antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of IFNG / Interferon Gamma could be seen inadipocytes in mesenchymal tissue, cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the tubules in kidney, fibroblasts in skin, glandular cells in the adrenal gland, appendix, cervix, uterine, duodenum, epididymis, gallbladder, parathyroid gland, rectum, seminal vesicle, small intestine and stomach, keratinocytes in skin, Langerhans in skin, Leydig cells in the testis, macrophages in lung, melanocytes in skin, myocytes in heart muscle and skeletal muscle, neuronal cells in the hippocampus, non-germinal center cells in the tonsil, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, smooth muscle cells in the smooth muscle, squamous epithelial cells in the oral mucosa and urothelial cells in the urinary bladder. We were unable to detect IFNG / Interferon Gamma in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of IFNG / Interferon Gamma expression as measured by anti-IFNG / Interferon Gamma antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - + - - - - - - - - - - - - -
IFNG Variability ++ ++ + ++ ++ + ++ + ++ + ++ + ++ + ++ + + + ++ +

Selected References

Choudhry N, Petry F, van Rooijen N, and McDonald V. 2012. J. of Infect. Disease. 206: 117-124. (in vivo neutralization)Cobb D and Smeltz RB. 2012. J. Immunol. 188: 3766-3773. (in vitro neutralization)Brown DM, Lee S, Garcia-Hernandez M, and Swain SL. 2012. J. Virol. 86: 6792-6803. (ELISpot - detection)Yu H, Karunakaran KP, Jiang X, Shen C, Andersen P, and Brunham RC. 2012. Infect. Immun. 80: 1510-1518. (ELISpot -detection)Kwon M-J, Ma J, Ding Y, Wang R, and Sun Z. 2012. J. Immunol. 188: 5887-5897. (in vitro induction of Th2 polarization)Barr TA, Shen P, Brown S, Lampropoulou V, Roch T, Lawrie S, Fan B, O’Connor RA, Anderton SM, Bar-Or Am Fillatreau S, and Gray D. 2012. J. Exp. Med. 209: 1001-1010. (Flow cytometry)Cardona AE, Restrepo BI, Jaramillo JM, and Teale JM. 1999. J. Immunol. 162: 995-1002. (Immunohistochemistry - frozen tissue)Kupfer A, Mosmann TR, and Kupfer H. 1991. Proc. Natl. Acad. Sci. 88: 775-779. (Immunofluorescence microscopy)

Limitations and Warranty

enQuire Bio's Mouse Anti-IFN gamma Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Mouse Anti-IFN gamma Antibody [XMG1.2]”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top