Mouse Anti-IL-12 / IL-23 p40 Antibody [C17.8]

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SKU: QAB96
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB96-PE-25ug
IL-12 p40 / IL-23 p40 General Information
Alternate Names
Interleukin-12, IL12 / Interleukin-23, IL23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Curated Database and Bioinformatic Data
Gene SymbolIl12b
Entrez Gene ID16160
Ensemble Gene IDENSMUSG00000004296
RefSeq Protein Accession(s)NP_001290173
RefSeq mRNA Accession(s)NM_008352, NM_001303244
RefSeq Genomic Accession(s)NC_000077,
UniProt ID(s)P43432, Q3ZAX5
UniGene ID(s)P43432, Q3ZAX5
Cosmic ID(s)Il12b
KEGG Gene ID(s)mmu:16160
General Description of IL-12 p40 / IL-23 p40.
The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target nave, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19; its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the receptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine. Please choose the appropriate format for each application.

Mouse Anti-IL-12 p40 / IL-23 p40 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-IL-12 p40 / IL-23 p40 Antibody Clone: C17.8
Clone C17.8 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

IL-12 p40 / IL-23 p40 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in IL-12 p40 / IL-23 p40 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of IL-12 p40 / IL-23 p40 could be seen in cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the colon, duodenum, gallbladder, small intestine and stomach, glial cells in the caudate nucleus, hepatocytes in liver, keratinocytes in skin, myocytes in skeletal muscle and neuronal cells in the caudate nucleus. We were unable to detect IL-12 p40 / IL-23 p40 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of IL-12 p40 / IL-23 p40 expression as measured by anti-IL-12 p40 / IL-23 p40 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - ++ - - - - - - - - -
IL12B Variability + + + + + + + + + + ++ + + + + + + + ++ +

Selected References

Dong H, Franklin NA, Roberts DJ, Yagita H, Glennie MJ and Bullock TNJ. 2012. J. Immunol. 188: 3829-3838. (in vivo blocking)Prabhakara R, Harro JM, Leid JG, Keegan AD, Prior ML, and Shirtliff ME. 2011. Infect. Immun. 79: 5010-5018. (in vivo blocking)Chmielewski M, Kopecky C, Hombach AA, and Abken H. 2011. Cancer Res. 71: 5697-5706. (ELISA)Lo C-H, Lee S-C, Wu P-Y, Pan W-Y et al. 2003. J. Immunol. 171: 600-607. (Immunoprecipitation)Belladonna ML, Renauld J-C, Bianchi R, Vacca C, Fallarino F, Orabona C, Fioretti MC, Grohmann, and Puccetti P. 2002. J. Immunol. 168: 5448-5454. (Western Blot)Ludviksson BR, Ehrhardt RO, and Strober W. 1999. J. Immunol. 163: 4349-4359. (Immunofluorescence microscopy - frozen tissue)Kato K, Shimozato O, Hoshi K, Wakimoto H, Hamada H, Yagita H, and Okumura K. 1996. Proc. Natl. Acad. Sci. 93:9085-9089. (Immunoprecipitation; ELISA)Wysocka M, Kubin M, Vieira LQ, Ozmen L, Garotta G, Scott P and Trinchieri G. 1995. Eur. J. Immunol. 25: 672-676. (Origination of clone; Immunoprecipitation, Western Blot)

Limitations and Warranty

enQuire Bio's Mouse Anti-IL-12 / IL-23 p40 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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