Mouse Anti-IL-12 p40 / IL-23 p40 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-IL-12 p40 / IL-23 p40 Antibody Clone: C17.8
Clone C17.8 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
IL-12 p40 / IL-23 p40 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in IL-12 p40 / IL-23 p40 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of IL-12 p40 / IL-23 p40 could be seen in cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the colon, duodenum, gallbladder, small intestine and stomach, glial cells in the caudate nucleus, hepatocytes in liver, keratinocytes in skin, myocytes in skeletal muscle and neuronal cells in the caudate nucleus. We were unable to detect IL-12 p40 / IL-23 p40 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of IL-12 p40 / IL-23 p40 expression as measured by anti-IL-12 p40 / IL-23 p40 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | - | - | - | - | - | - | - | - | ++ | - | - | - | - | - | - | - | - | - |
| IL12B Variability | + | + | + | + | + | + | + | + | + | + | ++ | + | + | + | + | + | + | + | ++ | + |
Selected References
Dong H, Franklin NA, Roberts DJ, Yagita H, Glennie MJ and Bullock TNJ. 2012. J. Immunol. 188: 3829-3838. (in vivo blocking)Prabhakara R, Harro JM, Leid JG, Keegan AD, Prior ML, and Shirtliff ME. 2011. Infect. Immun. 79: 5010-5018. (in vivo blocking)Chmielewski M, Kopecky C, Hombach AA, and Abken H. 2011. Cancer Res. 71: 5697-5706. (ELISA)Lo C-H, Lee S-C, Wu P-Y, Pan W-Y et al. 2003. J. Immunol. 171: 600-607. (Immunoprecipitation)Belladonna ML, Renauld J-C, Bianchi R, Vacca C, Fallarino F, Orabona C, Fioretti MC, Grohmann, and Puccetti P. 2002. J. Immunol. 168: 5448-5454. (Western Blot)Ludviksson BR, Ehrhardt RO, and Strober W. 1999. J. Immunol. 163: 4349-4359. (Immunofluorescence microscopy - frozen tissue)Kato K, Shimozato O, Hoshi K, Wakimoto H, Hamada H, Yagita H, and Okumura K. 1996. Proc. Natl. Acad. Sci. 93:9085-9089. (Immunoprecipitation; ELISA)Wysocka M, Kubin M, Vieira LQ, Ozmen L, Garotta G, Scott P and Trinchieri G. 1995. Eur. J. Immunol. 25: 672-676. (Origination of clone; Immunoprecipitation, Western Blot)
Limitations and Warranty
enQuire Bio's Mouse Anti-IL-12 / IL-23 p40 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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