Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Mammaglobin Antibody Clone: 304-1A5
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Breast Carcinoma
Cellular Localization of Antibody 304-1A5 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Purified mammaglobin recombinant protein, containing amino acids 15-93
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Mammaglobin Information for Pathologists
Summary:
Belongs to the family of small epithelial secretory proteins (secretoglobins) of the uteroglobin / Clara cell protein family. Uses by pathologists Highly specific for most breast cancers; useful to detect circulating or metastatic breast cancer (Arch Pathol Lab Med 2003;127:1330, Int J Clin Exp Pathol 2009;2:384, Mod Pathol 2010;23:654). Superior to GCDFP15 (Arch Pathol Lab Med 2008;132:239). Positive in endometrial and endocervical specimens (benign and malignant) (Int J Gynecol Pathol 2008;27:418).Notable Clinical Features:
While some expression is seen in normal breast, its expression is markedly increased in breast carcinoma (positive expression has been reported in 48 - 72.1% of mammary adenocarcinomas) and is directly correlated with a higher grade. Also, mammaglobin expression is said to be higher in lobular carcinomas than ductal carcinomas and in ER positive tumors. Case reports 62 year old woman with solitary uterine metastasis of invasive lobular carcinoma (J Med Case Rep 2015;9:47). Microscopic (histologic) imagesCommon Uses By Pathologists:
Highly specific for most breast cancers; useful to detect circulating or metastatic breast cancer (Arch Pathol Lab Med 2003;127:1330, Int J Clin Exp Pathol 2009;2:384, Mod Pathol 2010;23:654). Superior to GCDFP15 (Arch Pathol Lab Med 2008;132:239). Positive in endometrial and endocervical specimens (benign and malignant) (Int J Gynecol Pathol 2008;27:418). Has limited utility in medullary and metaplastic breast cancers (Am J Clin Pathol 2012;137:747). Useful to differentiate mammary analogue secretory carcinoma (MASC) from secretory carcinoma of breast (Hum Pathol 2013;44:1982).Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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