Immunohistochemical staining of p27Kip1  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

p27Kip1 [DCS-72.F6] Antibody (cGMP).

$ 135.00$ 725.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD166-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD166-0.5ml Size: 0.5 ml, Format: Concentrate $ 435.00
QIVD166-1ml Size: 1 ml, Format: Concentrate $ 725.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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Bioactivity (test results eg. IU/ml):

SKU: QIVD166-0.1ml
p27Kip1 General Information
Alternate Names
Molecular Weight
22.1 kDa
Chromosomal Location
p13.1 [chr: 12] [chr_start: 12715058] [chr_end: 12722369] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolCDKN1B
Entrez Gene ID1027
RefSeq Protein Accession(s)NP_004055
RefSeq mRNA Accession(s); NM_004064
RefSeq Genomic Accession(s)NG_016341; NC_000012
UniProt ID(s)P46527
PharmGKB ID(s)PA105
KEGG Gene ID(s)hsa:1027
Associated Diseases (KEGG IDs)Multiple endocrine neoplasia 4 (MEN4) [MIM:610755]: Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. {ECO:0000269|PubMed:17030811}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of p27Kip1 .
This antibody is specific to a protein of 27 kDa known as p27Kip1 a cell cycle regulatory mitotic inhibitor. This antibody recognizes an epitope between amino acids 83-204 of p27. This antibody does not show any cross-reactivity with other related mitotic inhibitors.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-p27Kip1 Antibody Clone: DCS-72.F6
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Colon carcinoma
Cellular Localization of Antibody DCS-72.F6 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with mouse recombinant p27 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

p27Kip1 Information for Pathologists


Cyclin dependent kinase inhibitor leading to cell cycle arrest in G1 phase. Member of Cip/Kip family of proteins. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung. Interpretation Nuclear stain.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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p27Kip1 [DCS-72.F6] Antibody (cGMP).