Immunohistochemical staining of PD1  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

PD1 [DBM15.5] Antibody (cGMP).

$ 135.00$ 645.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD176
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

Product NumberDescriptionPrice
QIVD176-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD176-0.5ml Size: 0.5 ml, Format: Concentrate $ 415.00
QIVD176-1ml Size: 1 ml, Format: Concentrate $ 575.00
QIVD176-6ml Size: 6 ml, Format: Predilute $ 255.00
QIVD176-25ml Size: 25 ml, Format: Predilute $ 645.00
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In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

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SKU: QIVD176-0.1ml
PD1 General Information
Alternate Names
Molecular Weight
31.6 kDa
Chromosomal Location
q37.3 [chr: 2] [chr_start: 241849884] [chr_end: 241858894] [strand: -1]; [chr: CHR_HSCHR2_3_CTG15] [chr_start: 241849881] [chr_end: 241858908] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolPDCD1
Entrez Gene ID5133
RefSeq Protein Accession(s)NP_005009
RefSeq mRNA Accession(s); NM_005018; XM_006712573; XM_017004293
RefSeq Genomic Accession(s)NT_187527; NC_000002; NG_012110
UniProt ID(s)Q15116
PharmGKB ID(s)PA33110
KEGG Gene ID(s)hsa:5133
Associated Diseases (KEGG IDs)Systemic lupus erythematosus 2 (SLEB2) [MIM:605218]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. {ECO:0000269|PubMed:12402038}. Disease susceptibility is associated with variations affecting the gene represented in this entry.
General Description of PD1 .
PD1, or CD279/PDCD1 (programmed cell death-1 protein), is a type I transmembrane receptor expressed on activated T-cells, B-cells, and myeloid cells. Engagement of PD1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. Anti-PDCD1 is a marker of angioimmunoblastic lymphoma.also pro¬duce responses in nonimmunogenic cancers such as non–small-cell lung and colon cancers, broadening their scope beyond classic immunogenic tumors like melanoma and renal cell cancer.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-PD1 Antibody Clone: DBM15.5
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Squamous lung carcinoma
Cellular Localization of Antibody DBM15.5 Staining: Cytoplasm and cell surface
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant full-length human PDCD1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

PD1 Information for Pathologists

Summary:

Also known as CD279. Coinhibitory receptor of lymphocytes / other immune system cells. Controls lymphocyte activation by providing negative signals in conjunction with signals from lymphocyte antigen receptors (Future Oncol 2011;7:929). Expressed by germinal center associated helper T cells; inhibits T cell activity (Hum Pathol 2009;40:1715). Expressed by CD8+ T cells, associated with CD8 activation (AIDS Res Hum Retroviruses 2012;28:465).

Common Uses By Pathologists:

Differentiate primary cutaneous CD4 small / medium sized pleomorphic T cell lymphoma and cutaneous pseudo-T cell lymphomas (PD-1 positive) from other cutaneous T cell lymphomas (usually PD-1 negative, Am J Surg Pathol 2012;36:109). PD-1+ rosettes of T cells around neoplastic cells is relatively specific for nodular lymphocyte predominant Hodgkin lymphoma (Hum Pathol 2009;40:1715). Microscopic (histologic) images Images hosted on PathOut server:. Contributed by GenomeMe:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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PD1 [DBM15.5] Antibody (cGMP).