Flow Cytometry of NCCIT Cells using Podocalyxin Monoclonal Antibody (3D3).
Flow Cytometry of NCCIT Cells using Podocalyxin Monoclonal Antibody (3D3).Western Blot of HeLa Lysate using Podocalyxin Monoclonal Antibody (3D3).Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Monoclonal Antibody (3D3).

Anti-Podocalyxin Antibody Clone 3D3

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5420-MSM1
Species: Human, Rabbit, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5420-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 5420-MSM1-P1
Podocalyxin General Information
Alternate Names
Podocalyxin-like protein 1, PODXL
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolPODXL
Entrez Gene ID5420
Ensemble Gene IDENSG00000128567
RefSeq Protein Accession(s)NP_001018121, NP_005388
RefSeq mRNA Accession(s)NM_005397 NM_001018111
RefSeq Genomic Accession(s)NC_000007, NC_018918, NG_042104
UniProt ID(s)O00592, Q96N83
UniGene ID(s)O00592, Q96N83
HGNC ID(s)9171
Cosmic ID(s)PODXL
KEGG Gene ID(s)hsa:5420
PharmGKB ID(s)PA33493
General Description of Podocalyxin.
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections, plays essential roles in kidney development, homeostasis, blood filtration, urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors, a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia,, other kidney pathology.

Human, Rabbit, and Rat Anti-Podocalyxin Antibody Product Attributes

Species: Human, Rabbit, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunoprecipitation (1-2ug of antibody/500ug protein), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Podocalyxin Antibody Clone: 3D3
Clone 3D3 Host and Isotype: Mouse IgG1
Anti-Human, Rabbit, and Rat Podocalyxin Positive Control Sample: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney
Cellular Localization of Antibody <3D3 Staining: Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A recombinant protein fragment containing the intracellular, transmembrane,, part of the extracellular domain of human podocalyxin
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Podocalyxin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-PODXL antibody staining is expected to be primarily localized to the plasma membrane, microtubule organizing center and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Podocalyxin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the cerebral cortex, glandular cells in the fallopian tube and cells in the glomeruli in kidney. More moderate antibody staining intensity was present in endothelial cells in the cerebral cortex, glandular cells in the fallopian tube and cells in the glomeruli in kidney. Low, but measureable presence of Podocalyxin could be seen inglandular cells in the breast, decidual cells in the placenta and glandular cells in the seminal vesicle. We were unable to detect Podocalyxin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Podocalyxin expression as measured by anti-Podocalyxin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - ++ - - - - - - - - - - - - - - -
PODXL Variability + + + + +++ ++ ++ + + + + ++ ++ + + + + ++ + +

Limitations and Warranty

enQuire Bio's Podocalyxin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Podocalyxin Antibody Clone 3D3