Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:75 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Retinoblastoma Gene Protein Antibody Clone: 1F8
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Colon carcinoma
Cellular Localization of Antibody 1F8 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant human Rb protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Retinoblastoma Gene Protein Information for Pathologists
Summary:
Retinoblastoma Gene Product (Rb) is a 110-114 kDa nuclear protein that plays a crucial role in cell cycle progression by regulating cell cycle arrest at G1-S. It is encoded by a tumor suppressor gene at 13q14. Rb is present in germline mutations or loss predispose to retinoblastoma and osteosarcoma. Somatic mutations cause various tumors and specific point mutations my inhibit Rb-1 and c-myc binding. For thyroid neoplasms, follicular adenomas were usually positive, follicular and papillary carcinomas were usually negative.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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