Human Anti-Spectrin beta III Antibody Product Attributes
Tested Applications: Western Blot.
Anti-Spectrin beta III Antibody Clone: SPTBN2/1582
Clone SPTBN2/1582 Host and Isotype: Mouse IgG2a kappa
Anti-Human Spectrin beta III Positive Control Sample: HeLa cells. Pancreas or Liver.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human SPTBN2 fragment (aa356-475)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Spectrin beta III Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-SPTBN2 antibody staining is expected to be primarily localized to the cell junctions and cytosol.
Observed Antibody Staining Data By Tissue Type:Variations in Spectrin beta III antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, Purkinje cells in the cerebellum, neuropil in cerebral cortex, glandular cells in the cervix, uterine, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the prostate, keratinocytes in skin and epidermal cells in the skin. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, Purkinje cells in the cerebellum, neuropil in cerebral cortex, glandular cells in the cervix, uterine, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the prostate, keratinocytes in skin and epidermal cells in the skin. Low, but measureable presence of Spectrin beta III could be seen inglandular cells in the breast, respiratory epithelial cells in the bronchus, glandular cells in the endometrium, epididymis and gallbladder, hepatocytes in liver, macrophages in lung, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, Langerhans in skin, cells in the red pulp in spleen and cells in the seminiferous ducts in testis. We were unable to detect Spectrin beta III in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Spectrin beta III expression as measured by anti-Spectrin beta III antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's Spectrin beta III Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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