Western Blot of HeLa Cell Lysateusing SUMO-2 Monoclonal Antibody (SM23/496)

Anti-SUMO-2/3 Antibody Clone SM23/496

Name: Anti-SUMO-2/3 Antibody Clone SM23/496
Brand: enQuire Bio
Price: 488 USD
Availability: InStock

$ 429.00

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SKU: 6613-MSM1
Species: Human and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
6613-MSM1-P1	Size: 100 ug, Tag: Unconjugated

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In Stock.

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 6613-MSM1-P1

SUMO-2/3 General Information
Alternate Names
Recombinant human SUMO2 protein, anti-Recombinant human SUMO2 protein antibody
Molecular Weight
11-13kDa
Chromosomal Location
17q25.1 (SUMO-2) & 21q22.3 (SUMO-3)
Curated Database and Bioinformatic Data
Gene Symbol	SUMO2 & SUMO3
Entrez Gene ID	6613
Ensemble Gene ID	ENSG00000188612
RefSeq Protein Accession(s)	NP_001005849, NP_008868
RefSeq mRNA Accession(s)	NM_001005849, NM_006937
RefSeq Genomic Accession(s)	NC_000017, NC_018928
UniProt ID(s)	P55855, A0A024R8S3, P61956
UniGene ID(s)	P55855, A0A024R8S3, P61956
HGNC ID(s)	11125
Cosmic ID(s)	SUMO2
KEGG Gene ID(s)	hsa:6613
PharmGKB ID(s)	PA134914683
General Description of SUMO-2/3.
This MAb reacts with both SUMO-2, SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2, 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, E3 cascade enzymes for conjugation. However, SUMO, ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis, protein stability. The unconjugated SUMO-1, 2, 3 proteins localize to the nuclear membrane, nuclear bodies, cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML, RanGap1. SUMO-2, 3 contribute to a greater percentage of protein modification than does SUMO-1, unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation, may be critical in the onset or progression of AlzheimerÂ’s disease.

Human and Broad Species Reactivity Anti-SUMO-2/3 Antibody Product Attributes

Species: Human and Broad Species Reactivity

Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-SUMO-2/3 Antibody Clone: SM23/496

Clone SM23/496 Host and Isotype: Mouse IgG1 kappa

Anti-Human and Broad Species Reactivity SUMO-2/3 Positive Control Sample: HeLa cells or breast carcinoma

Cellular Localization of Antibody Nuclear (SUMO-2); Cytoplasmic (SUMO-3)

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Mouse / IgG1, kappa

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

SUMO-2/3 Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's SUMO-2/3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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