Immunohistochemical staining of TdT  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

TdT [EP266] Antibody (cGMP).

$ 135.00$ 745.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD195
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Rabbit IgG

Product NumberDescriptionPrice
QIVD195-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD195-0.5ml Size: 0.5 ml, Format: Concentrate $ 465.00
QIVD195-1ml Size: 1 ml, Format: Concentrate $ 745.00
QIVD195-6ml Size: 6 ml, Format: Predilute $ 335.00
QIVD195-10ml Size: 10 ml, Format: Predilute $ 535.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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SKU: QIVD195-0.1ml
TdT General Information
Alternate Names
Molecular Weight
58.5 kDa
Chromosomal Location
q24.1 [chr: 10] [chr_start: 96304409] [chr_end: 96338564] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolDNTT
Entrez Gene ID1791
RefSeq Protein Accession(s)NP_004079; NP_001017520
RefSeq mRNA Accession(s)NM_001017520; NM_004088
RefSeq Genomic Accession(s)NC_000010
UniProt ID(s)P04053
PharmGKB ID(s)PA27449
KEGG Gene ID(s)hsa:1791
General Description of TdT .
Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase that contributes to the generation of junctional diversity in antigen receptors of immature lymphocytes. TdT is expressed in lymphoid precursors of B- and T-cell lineage in thymus and bone marrow. Foci of TdT positive cells may be observed in peripheral lymphoid tissues. TdT is also present in malignant tumors of lymphoblastic lineage and thymoma. It is sensitive and specific marker for lymphoblastic lymphoma/leukemia.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-TdT Antibody Clone: EP266
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Tonsil, Thymus
Cellular Localization of Antibody EP266 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues of human TdT protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

TdT Information for Pathologists

Summary:

Terminal deoxynucleotidyl transferase; also called terminal transferase. Nuclear DNA polymerase in thymic and small number of bone marrow cortical lymphocytes. Catalyzes addition of deoxynucleotides to 3' OH ends of oligonucleotides or polydeoxynucleotides. Performs DNA synthesis using only single-stranded DNA as the nucleic acid substrate (Biochim Biophys Acta 2010;1804:1151). Used during immunoglobulin and T cell receptor rearrangement, adds diversity to antibody recognition sites.

Common Uses By Pathologists:

Interpretation: nuclear stain. Marker of B or T cell acute lymphoblastic leukemia/lymphoma (only rarely negative, Arch Pathol Lab Med 2000;124:92). Microscopic (histologic) images Image hosted on PathOut server:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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TdT [EP266] Antibody (cGMP).