Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).
Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).Western Blot of HepG2, HeLa and 3T3 Cell Lysate using Topo II alpha, Monoclonal Antibody (TOP2A/1361).

Anti-Topoisomerase II alpha Antibody Clone TOP2A/1361

$ 199.00$ 459.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7153-MSM1
Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7153-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
7153-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
7153-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
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SKU: 7153-MSM1-P0
Topoisomerase II alpha General Information
Alternate Names
DNA topoisomerase 2-alpha, TOP2A
Molecular Weight
170kDa
Chromosomal Location
17q21.2
Curated Database and Bioinformatic Data
Gene SymbolTOP2A
Entrez Gene ID7153
Ensemble Gene IDENSG00000131747
RefSeq Protein Accession(s)XP_005257689, NP_001058, XP_011523467
RefSeq mRNA Accession(s)XM_005257632, XM_011525165, NM_001067,
RefSeq Genomic Accession(s)NC_018928, NC_000017, NG_027678
UniProt ID(s)P11388
UniGene ID(s)P11388
HGNC ID(s)11989
Cosmic ID(s)TOP2A
KEGG Gene ID(s)hsa:7153
PharmGKB ID(s)PA354
General Description of Topoisomerase II alpha.
This MAb recognizes a 170kDa protein, which is identified as topoisomerase II?. ?? shows no cross-reaction with Topoisomerase II? or I. Topo II? plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- ;M-phases in transformed and developmentally regulated normal cells. Topo II? is also implicated in drug resistance of tumor cells.

Human and Mouse Anti-Topoisomerase II alpha Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Topoisomerase II alpha Antibody Clone: TOP2A/1361
Clone TOP2A/1361 Host and Isotype: Mouse IgG2b kappa
Anti-Human and Mouse Topoisomerase II alpha Positive Control Sample: HeLa cells or Tonsil
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Topoisomerase II alpha Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TOP2A antibody staining is expected to be primarily localized to the nucleus and nucleoli. There is variability in either the signal strength or the localization of signal in nucleoli and nucleus from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Topoisomerase II alpha antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, germinal center cells in the lymph node, cells in the seminiferous ducts in testis and germinal center cells in the tonsil. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, germinal center cells in the lymph node, cells in the seminiferous ducts in testis and germinal center cells in the tonsil. Low, but measureable presence of Topoisomerase II alpha could be seen inglandular cells in the adrenal gland and breast, respiratory epithelial cells in the bronchus, cells in the endometrial stroma in endometrium, glandular cells in the fallopian tube and gallbladder, pneumocytes in lung, respiratory epithelial cells in the nasopharynx and glandular cells in the parathyroid gland and seminal vesicle. We were unable to detect Topoisomerase II alpha in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Topoisomerase II alpha expression as measured by anti-Topoisomerase II alpha antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ ++ ++ +++ ++ +++ ++ ++ +++ ++ ++ ++ +++ ++ +++ ++ +++
TOP2A Variability ++ ++ + + ++ ++ + ++ + ++ ++ ++ ++ + ++ ++ ++ + + ++

Limitations and Warranty

enQuire Bio's Topoisomerase II alpha Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Topoisomerase II alpha Antibody Clone TOP2A/1361

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