Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Monoclonal Antibody (ACP5/17)
Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Monoclonal Antibody (ACP5/17)

Anti-TRAcP Antibody Clone ACP5/1070

$ 298.00
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SKU: 54-MSM1
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
54-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 54-MSM1-P1
TRAcP General Information
Alternate Names
Tartrate-resistant acid phosphatase, TRAP, TRAPase, acid phosphatase 5, tartrate resistant, ACP5
Molecular Weight
35kDa
Chromosomal Location
19p13.2
Curated Database and Bioinformatic Data
Gene SymbolACP5
Entrez Gene ID54
Ensemble Gene IDENSG00000102575
RefSeq Protein Accession(s)NP_001104506, NP_001104505, NP_001308952, XP_011526371, NP_001104504, XP_005259995, NP_001602
RefSeq mRNA Accession(s)NM_001611, XM_005259938, NM_001322023, XM_011528069, NM_001111034, NM_001111035, NM_001111036
RefSeq Genomic Accession(s)NG_028127, NC_018930, NC_000019
UniProt ID(s)P13686, A0A024R7F8
UniGene ID(s)P13686, A0A024R7F8
HGNC ID(s)124
Cosmic ID(s)ACP5
KEGG Gene ID(s)hsa:54
PharmGKB ID(s)PA24448
General Description of TRAcP.
It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a, 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages, dendritic cells, increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts, elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol, orthophosphate. It is the most basic of the acid phosphatases, is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme, is activated by proteolytic cleavage, reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons, endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gauchers Disease, HIV-induced Encephalopathy, Osteoclastoma, in osteoporosis, metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity, specificity. Other cells stained with this antibody are tissue macrophages, osteoclasts.

Human, Mouse, and Rat Anti-TRAcP Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-TRAcP Antibody Clone: ACP5/1070
Clone ACP5/1070 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Mouse, and Rat TRAcP Positive Control Sample: HepG2, 293T, K562 or RPMI-8226 Cells. Spleen from Hairy Cell Leukemia (HCL) patient.
Cellular Localization of Antibody Cytoplasmic (Lysosomes)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ACP5 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

TRAcP Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ACP5 antibody staining is expected to be primarily localized to the cytosol.

Limitations and Warranty

enQuire Bio's TRAcP Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-TRAcP Antibody Clone ACP5/1070

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