FFPE human ovarian carcinoma sections stained with 100 ul anti-VEGF (clone SPM225) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Monoclonal Antibody (SPM225).

Anti-VEGF Antibody Clone SPM225

Name: Anti-VEGF Antibody Clone SPM225
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 7422-MSM1X
Species: Human, Mouse, Rat, Rabbit, and Canine
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
7422-MSM1X-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 7422-MSM1X-P1

VEGF General Information
Alternate Names
Vascular endothelial growth factor A, VEGF-A, VEGFA
Molecular Weight
19-22kDa (reducing), 38kDa-44kDa (non-reducing)
Chromosomal Location
6p12
Curated Database and Bioinformatic Data
Gene Symbol	VEGFA
Entrez Gene ID	7422
Ensemble Gene ID	ENSG00000112715
RefSeq Protein Accession(s)	NP_001165094, NP_001165101, NP_001273973, NP_003367, NP_001020541, NP_001165093, NP_001165095, NP_001165098, NP_001020538, NP_001028928, NP_001165097, NP_001165099, NP_001303939, NP_001020539, NP_001165100, NP_001191314, NP_001020537, NP_001020540, NP_001165096, NP_001191313
RefSeq mRNA Accession(s)	NM_001025367, NM_001025369, NM_001171627, NM_001204384, NM_001317010, NM_003376 NM_001025368, NM_001171623, NM_001287044, NM_001033756, NM_001171628, NM_001171626, NM_001171630, NM_001171625, NM_001171629, NM_001025366, NM_001025370, NM_001171622, NM_001204385, NM_001171624
RefSeq Genomic Accession(s)	NC_000006, NC_018917, NG_008732
UniProt ID(s)	A0A024RD37, A0A024RD33, A0A087X1L1, A0A0Y0IMM4, A0A087WUD8, A2A2V4, A0A087X0B2, A0A087WTP4, P15692
UniGene ID(s)	A0A024RD37, A0A024RD33, A0A087X1L1, A0A0Y0IMM4, A0A087WUD8, A2A2V4, A0A087X0B2, A0A087WTP4, P15692
HGNC ID(s)	12680
Cosmic ID(s)	VEGFA
KEGG Gene ID(s)	hsa:7422
PharmGKB ID(s)	PA37302
General Description of VEGF.
This MAb recognizes proteins of 19-22kDa (reducing), 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-,, 121-amino acid residues. The smaller two isoforms, VEGF165, VEGF121, are secreted proteins, act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression, metastasis.

Human, Mouse, Rat, Rabbit, and Canine Anti-VEGF Antibody Product Attributes

Species: Human, Mouse, Rat, Rabbit, and Canine

Tested Applications: Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-VEGF Antibody Clone: SPM225

Clone SPM225 Host and Isotype: Mouse IgG1 kappa

Anti-Human, Mouse, Rat, Rabbit, and Canine VEGF Positive Control Sample: Tumor cells in astrocytomas, breast or ovarian carcinomas.

Cellular Localization of Antibody Cytoplasmic, Cell Surface, Extracellular (Secreted)

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Human VEGF189 recombinant protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

VEGF Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in VEGF antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in respiratory epithelial cells in the bronchus, glandular cells in the colon, duodenum, endometrium and gallbladder, hepatocytes in liver, macrophages in lung, exocrine glandular cells in the pancreas, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland, seminal vesicle and small intestine, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis, glandular cells in the thyroid gland, squamous epithelial cells in the tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in respiratory epithelial cells in the bronchus, glandular cells in the colon, duodenum, endometrium and gallbladder, hepatocytes in liver, macrophages in lung, exocrine glandular cells in the pancreas, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland, seminal vesicle and small intestine, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis, glandular cells in the thyroid gland, squamous epithelial cells in the tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of VEGF could be seen in cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex, neuropil in cerebral cortex, endothelial cells in the colon, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, cells in the red pulp in spleen and cells in the white pulp in spleen. We were unable to detect VEGF in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of VEGF expression as measured by anti-VEGF antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	++	++	++	+++	+++	++	++	+++	+++	+	++	++	++	+++	++	+++	++	-	++	++
VEGFA Variability	++	++	++	+	++	+++	++	+	+	++	++	+++	++	++	++	++	++	++	++	++

Limitations and Warranty

enQuire Bio's VEGF Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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