SDS-PAGE Analysis Purified Calnexin Mouse Monoclonal Antibody (CANX/1543). Confirmation of Integrity and Purity of Antibody.
SDS-PAGE Analysis Purified Calnexin Mouse Monoclonal Antibody (CANX/1543). Confirmation of Integrity and Purity of Antibody.Western Blot of Analysis of Human Kidney Lysate using Calnexin Mouse Monoclonal Antibody (CANX/1543).Analysis of Protein Array containing more than 19,000 full-length human proteins using Calnexin Mouse Monoclonal Antibody (CANX/1543). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Western Blot of Analysis of PANC1 and MCF-7 Cell Lysate using Calnexin Mouse Monoclonal Antibody (CANX/1543).

Calnexin (Endoplasmic Reticulum Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 821-MSM3
Species: Human
Tested Applications: ELISA, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 821-MSM3-P0
Calnexin (Endoplasmic Reticulum Marker) General Information
Alternate Names
Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90
Molecular Weight
90kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol821
Entrez Gene IDCANX
UniProt ID(s)P27824
UniGene ID(s)Hs567968
COSMIC ID Link(s)CANX
KEGG Gene ID(s)hsa:821
General Description of Calnexin (Endoplasmic Reticulum Marker).
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. 

Human Anti-Calnexin (Endoplasmic Reticulum Marker) Antibody Product Attributes

Species: Human
Tested Applications: ELISA, WB, IHC.
Clonality: Monoclonal
Anti-Calnexin (Endoplasmic Reticulum Marker) Antibody Clone: CANX/1543
Clone CANX/1543 Host and Isotype: Mouse IgG1, kappa
Anti-Human Calnexin (Endoplasmic Reticulum Marker) Positive Control Sample: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
Cellular Localization of Antibody CANX/1543 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant N-terminal fragment of human Calnexin protein (apprx. aa 1-300) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Calnexin (Endoplasmic Reticulum Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CANX antibody staining is expected to be primarily localized to the endoplasmic reticulum.

Observed Antibody Staining Data By Tissue Type:

Variations in Calnexin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, cells in the tubules in kidney, endothelial cells in the colon, exocrine glandular cells in the pancreas, fibroblasts in skin and mesenchymal tissue, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, macrophages in lung, melanocytes in skin, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, non-germinal center cells in the lymph node and tonsil, peripheral nerve/ganglion in colon, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, cells in the tubules in kidney, endothelial cells in the colon, exocrine glandular cells in the pancreas, fibroblasts in skin and mesenchymal tissue, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, macrophages in lung, melanocytes in skin, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, non-germinal center cells in the lymph node and tonsil, peripheral nerve/ganglion in colon, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Calnexin could be seen inmyocytes in skeletal muscle. We were unable to detect Calnexin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Calnexin expression as measured by anti-Calnexin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ +++ ++ +++ +++ +++ +++ +++ ++ +++ +++ +++ +++ +++ +++ ++ +++ +++ +++ +++
CANX Variability + + +++ + + + ++ ++ ++ ++ ++ ++ + + + ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's product, Calnexin (Endoplasmic Reticulum Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Calnexin (Endoplasmic Reticulum Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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