Analysis of Protein Array containing >19,000 full-length human proteins using Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/798) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Analysis of Protein Array containing >19,000 full-length human proteins using Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/798) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.SDS-PAGE Analysis Purified Chromogranin A Mouse Recombinant Monoclonal Ab (rCHGA/798).Confirmation of Purity and Integrity of Antibody.

Chromogranin A / CHGA (Neuroendocrine Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1113-MSM17
Species: Human, Mouse and Rat
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1113-MSM17-P0 Size: 20 ug, Tag: Unconjugated $ 158.00
1113-MSM17-P1 Size: 100 ug, Tag: Unconjugated $ 348.00
1113-MSM17-P1ABX Size: 100 ug, Tag: Unconjugated $ 348.00
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SKU: 1113-MSM17-P0
Chromogranin A / CHGA (Neuroendocrine Marker) General Information
Alternate Names
Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Pituitary Secretory Protein I; SP-I; Vasostatin I or II
Molecular Weight
68-75kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol1113
Entrez Gene IDCHGA
UniProt ID(s)P10645
UniGene ID(s)Hs150793
COSMIC ID Link(s)CHGA
KEGG Gene ID(s)hsa:1113
General Description of Chromogranin A / CHGA (Neuroendocrine Marker).
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Human, Mouse and Rat Anti-Chromogranin A / CHGA (Neuroendocrine Marker) Antibody Product Attributes

Species: Human, Mouse and Rat
Tested Applications: IHC.
Clonality: Monoclonal
Anti-Chromogranin A / CHGA (Neuroendocrine Marker) Antibody Clone: rCHGA/798
Clone rCHGA/798 Host and Isotype: Mouse IgG1, kappa
Anti-Human, Mouse and Rat Chromogranin A / CHGA (Neuroendocrine Marker) Positive Control Sample: PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.
Cellular Localization of Antibody rCHGA/798 Staining: Finely granular cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human CHGA protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Chromogranin A / CHGA (Neuroendocrine Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CHGA antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Chromogranin A / CHGA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the parathyroid gland and islets of Langerhans in pancreas. More moderate antibody staining intensity was present in glandular cells in the parathyroid gland and islets of Langerhans in pancreas. Low, but measureable presence of Chromogranin A / CHGA could be seen inneuronal cells in the caudate nucleus, cerebral cortex and hippocampus, respiratory epithelial cells in the bronchus and neuronal cells in the caudate nucleus and cerebral cortex and hippocampus. We were unable to detect Chromogranin A / CHGA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Chromogranin A / CHGA expression as measured by anti-Chromogranin A / CHGA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - +++ - - - - - - - - - - - - - - - - - -
CHGA Variability + + + + ++ + ++ + + + + + + + + ++ ++ + + +

Limitations and Warranty

enQuire Bio's product, Chromogranin A / CHGA (Neuroendocrine Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Chromogranin A / CHGA (Neuroendocrine Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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