SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2341).Confirmation of Integrity and Purity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (GFR/2341) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Mouse Monoclonal Antibody (GFR/2341).

EGFR (Epidermal Growth Factor Receptor) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: EGFR (Epidermal Growth Factor Receptor) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 1956-MSM11-P0
Availability: InStock

$ 228.00 – $ 488.00

Please Select Product Options Below To View The Catalog Number.

SKU: 1956-MSM11
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
1956-MSM11-P0	Size: 20 ug, Tag: Unconjugated	$ 199.00
1956-MSM11-P1	Size: 100 ug, Tag: Unconjugated	$ 429.00
1956-MSM11-P1ABX	Size: 100 ug, Tag: Unconjugated	$ 429.00

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In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 1956-MSM11-P0

EGFR (Epidermal Growth Factor Receptor) General Information
Alternate Names
Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5
Molecular Weight
~170kDa (wild type) and ~145kDa (vIII variant)
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	1956
Entrez Gene ID	EGFR
UniProt ID(s)	P00533
UniGene ID(s)	Hs488293
COSMIC ID Link(s)	EGFR
KEGG Gene ID(s)	hsa:1956
General Description of EGFR (Epidermal Growth Factor Receptor).
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Human Anti-EGFR (Epidermal Growth Factor Receptor) Antibody Product Attributes

Species: Human

Tested Applications: IHC.

Clonality: Monoclonal

Anti-EGFR (Epidermal Growth Factor Receptor) Antibody Clone: GFR/2341

Clone GFR/2341 Host and Isotype: Mouse IgG2b, kappa

Anti-Human EGFR (Epidermal Growth Factor Receptor) Positive Control Sample: A431 cells. Placenta, Breast, Colon or Bladder cancer

Cellular Localization of Antibody GFR/2341 Staining: Cell Surface

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Recombinant fragment of human EGFR protein (apprx. aa 344-492) (Please call for additional information.)

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

EGFR (Epidermal Growth Factor Receptor) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-EGFR antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in EGFR antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of EGFR could be seen inhepatocytes in liver, Leydig cells in the testis, melanocytes in skin, myocytes in skeletal muscle, squamous epithelial cells in the tonsil and vagina and urothelial cells in the urinary bladder. We were unable to detect EGFR in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of EGFR expression as measured by anti-EGFR antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
EGFR Variability	+	+	+	+	+	++	+	++	+	+	+	+	+	+	+	+	+	+	+	+

Limitations and Warranty

enQuire Bio's product, EGFR (Epidermal Growth Factor Receptor) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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EGFR (Epidermal Growth Factor Receptor) Ultraspecific Antibody Tested against >20,000 Human Proteins