Human Anti-Filaggrin (Keratinocyte Differentiation Marker) Antibody Product Attributes
Tested Applications: IHC.
Anti-Filaggrin (Keratinocyte Differentiation Marker) Antibody Clone: FLG/1945
Clone FLG/1945 Host and Isotype: Mouse IgG2b, kappa
Anti-Human Filaggrin (Keratinocyte Differentiation Marker) Positive Control Sample: U-251-MG cells or Skin
Cellular Localization of Antibody FLG/1945 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant human Filaggrin protein fragment (apprx. aa 998-1104) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.
Filaggrin (Keratinocyte Differentiation Marker) Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-FLG antibody staining is expected to be primarily localized to the vesicles.
Observed Antibody Staining Data By Tissue Type:
Variations in Filaggrin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin. More moderate antibody staining intensity was present in epidermal cells in the skin. Low, but measureable presence of Filaggrin could be seen infibroblasts in skin, melanocytes in skin, squamous epithelial cells in the cervix and uterine. We were unable to detect Filaggrin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's product, Filaggrin (Keratinocyte Differentiation Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.