Data from SDS-PAGE analysis of Anti-GAD1 antibody (Clone GAD1/2563). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

GAD1 / GAD67 (GABAergic Neuronal Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 228.00$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2571-MSM3
Species: Human
Tested Applications: ELISA, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2571-MSM3-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
2571-MSM3-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
2571-MSM3-P1ABX Size: 100 ug, Tag: Unconjugated $ 429.00
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SKU: 2571-MSM3-P0
GAD1 / GAD67 (GABAergic Neuronal Marker) General Information
Alternate Names
67kDa glutamic acid decarboxylase; CPSQ1; DCE1; GAD67; GAD1; Glutamate decarboxylase 1; SCP
Molecular Weight
67kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol2571
Entrez Gene IDGAD1
UniProt ID(s)Q99259
UniGene ID(s)Hs420036
COSMIC ID Link(s)GAD1
KEGG Gene ID(s)hsa:2571
General Description of GAD1 / GAD67 (GABAergic Neuronal Marker).
This MAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Human Anti-GAD1 / GAD67 (GABAergic Neuronal Marker) Antibody Product Attributes

Species: Human
Tested Applications: ELISA, WB, IHC.
Clonality: Monoclonal
Anti-GAD1 / GAD67 (GABAergic Neuronal Marker) Antibody Clone: GAD1/2563
Clone GAD1/2563 Host and Isotype: Mouse IgG2b, kappa
Anti-Human GAD1 / GAD67 (GABAergic Neuronal Marker) Positive Control Sample: K-562 or HEK293 Cells. Pancreas
Cellular Localization of Antibody GAD1/2563 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant human GAD1 (GAD67) protein fragment (apprx. aa 72-135) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

GAD1 / GAD67 (GABAergic Neuronal Marker) Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, GAD1 / GAD67 (GABAergic Neuronal Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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GAD1 / GAD67 (GABAergic Neuronal Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-GAD1 Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/GAD1 IHC human (GAD1 2563)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-GAD1(GAD1 2563) Gel-191x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-2571-MSM3-P0-anti-GAD1-GAD67-GABAergic-Neuronal-Marker-antibody-1-300x120.jpeg