FFPE human pancreas sections stained with 100 ul anti-GAD2 (clone GAD2/1960) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-GAD2 antibody (Clone GAD2/1960). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD2 (GAD65) Mouse Monoclonal Antibody (GAD2/1960) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

GAD2 / GAD65 (GABAergic Neuronal Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 228.00$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2572-MSM1
Species: Human
Tested Applications: ELISA, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2572-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
2572-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
2572-MSM1-P1ABX Size: 100 ug, Tag: Unconjugated $ 488.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 2572-MSM1-P0
GAD2 / GAD65 (GABAergic Neuronal Marker) General Information
Alternate Names
DCE2; GAD2; Glutamic Acid Decarboxylase 2 (Pancreas); Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65kDa); Glutamic Acid Decarboxylase 65 (GAD65)
Molecular Weight
65kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol2572
Entrez Gene IDGAD2
UniProt ID(s)Q05329
UniGene ID(s)Hs231829
COSMIC ID Link(s)GAD2
KEGG Gene ID(s)hsa:2572
General Description of GAD2 / GAD65 (GABAergic Neuronal Marker).
This MAb recognizes a protein of 65kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Human Anti-GAD2 / GAD65 (GABAergic Neuronal Marker) Antibody Product Attributes

Species: Human
Tested Applications: ELISA, WB, IHC.
Clonality: Monoclonal
Anti-GAD2 / GAD65 (GABAergic Neuronal Marker) Antibody Clone: GAD2/1960
Clone GAD2/1960 Host and Isotype: Mouse IgG2b, kappa
Anti-Human GAD2 / GAD65 (GABAergic Neuronal Marker) Positive Control Sample: Pancreas or Brain.
Cellular Localization of Antibody GAD2/1960 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant human GAD2 (GAD65) protein fragment (apprx. aa 6-99) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

GAD2 / GAD65 (GABAergic Neuronal Marker) Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, GAD2 / GAD65 (GABAergic Neuronal Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “GAD2 / GAD65 (GABAergic Neuronal Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Tested against >20,000 Human Proteins

), and application (e.g. IHC, Flow Cytometry, Western Blot) in your title."/>

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

GAD2 / GAD65 (GABAergic Neuronal Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-GAD2 Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/GAD2 IHC human Pancreas (GAD2 1960)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-GAD2(GAD2 1960) Gel.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-2572-MSM1-P0-anti-GAD2-GAD65-GABAergic-Neuronal-Marker-antibody-1-300x120.jpeg