Analysis of Protein Array containing more than 19,000 full-length human proteins using p63 Mouse Monoclonal Antibody (TP63/2427).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Analysis of Protein Array containing more than 19,000 full-length human proteins using p63 Mouse Monoclonal Antibody (TP63/2427).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

p63 (Squamous, Basal & Myoepithelial Cell Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 8626-MSM7
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 8626-MSM7-P0
p63 (Squamous, Basal & Myoepithelial Cell Marker) General Information
Alternate Names
Amplified in squamous cell carcinoma (AIS); Chronic ulcerative stomatitis protein (CUSP); EEC3; Keratinocyte transcription factor KET; LMS; NBP; p40; P51/P63; p53 like transcription factor; p53-related protein p63; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73; TP73L; Transformation-related protein 63; Trp53rp1; Trp6;3; Tumor protein 63; Tumor protein p53-like; tumor protein p73-like
Molecular Weight
63kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol8626
Entrez Gene IDTP63
UniProt ID(s)Q9H3D4
UniGene ID(s)Hs137569
COSMIC ID Link(s)TP63
KEGG Gene ID(s)hsa:8626
General Description of p63 (Squamous, Basal & Myoepithelial Cell Marker).
p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.

Human Anti-p63 (Squamous, Basal & Myoepithelial Cell Marker) Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-p63 (Squamous, Basal & Myoepithelial Cell Marker) Antibody Clone: TP63/2427
Clone TP63/2427 Host and Isotype: Mouse IgG2b, kappa
Anti-Human p63 (Squamous, Basal & Myoepithelial Cell Marker) Positive Control Sample: HEK293 cells or Prostate Carcinoma or Lung or bladder squamous cell carcinoma
Cellular Localization of Antibody TP63/2427 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human p63 protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

p63 (Squamous, Basal & Myoepithelial Cell Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TP63 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in p63 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. Low, but measureable presence of p63 could be seen inglandular cells in the breast, cervix, uterine. We were unable to detect p63 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p63 expression as measured by anti-p63 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - ++ - - - +++ - ++ - - + - - - +++ - + - +++
TP63 Variability + ++ ++ ++ ++ + + ++ ++ + ++ ++ + + + + + ++ ++ +

Limitations and Warranty

enQuire Bio's product, p63 (Squamous, Basal & Myoepithelial Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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p63 (Squamous, Basal & Myoepithelial Cell Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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