Recombinant Human bFGF / FGF2 Protein

$ 69.00$ 939.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP5269
Species: Human
Applications: Bioactive
Available Tags: Untagged
Host: E. coli
Purity: Greater than 95% as determined by SDS-PAGE.
Length (Amino Acids): 155

Product NumberDescriptionPrice
QP5270-10ug Tag: Untagged (Native Protein), Size: 10 ug $ 79.00
QP5270-50ug Tag: Untagged (Native Protein), Size: 50 ug $ 189.00
QP5270-500ug Tag: Untagged (Native Protein), Size: 100 ug $ 259.00
QP5270-500ug Tag: Untagged (Native Protein), Size: 500 ug $ 559.00
QP5270-1mg Tag: Untagged (Native Protein), Size: 1 mg $ 939.00
QP5269-10ug Tag: Untagged (Native Protein), Size: 10 ug $ 69.00
QP5269-50ug Tag: Untagged (Native Protein), Size: 50 ug $ 169.00
QP5269-100ug Tag: Untagged (Native Protein), Size: 100 ug $ 239.00
QP5269-500ug Tag: Untagged (Native Protein), Size: 500 ug $ 509.00
QP5269-1mg Tag: Untagged (Native Protein), Size: 1 mg $ 879.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP5269-50ug
Recombinant Human bFGF / FGF2 Protein General Information
Alternate Names
bFGF, FGF2, FGF-2, HBGF-2, BFGF, FGFB
Curated Database and Bioinformatic Data
Gene SymbolFGF2
Entrez Gene ID2247
Ensemble Gene IDENSG00000138685
RefSeq Protein Accession(s)NP_001997.5
RefSeq mRNA Accession(s)NM_002006.4
UniProt ID(s)P09038
UniGene ID(s)Hs.284244
HGNC ID(s)HGNC:3676
COSMIC ID Link(s)FGF2
KEGG Gene ID(s)hsa:2247
PharmGKB ID(s)PA28115
General Description of Recombinant Human bFGF / FGF2 Protein.
Basic fibroblast growth factor (bFGF), also known as FGF2, is expressed by endothelial cells, has mitogenic functions, and is an important growth factor in angiogenesis. Like other members of the fibroblast growth factor family, bFGF also promotes sell survival. bFGF has cardioprotective functions. bFGF is a critical component of embryonic stem cell culture systems to maintain cells in an undifferentiated state. Recombinant bFGF is the full 154 amino acid protein encoded by the human FGF2 gene. There are no detectable differences in biological activity between the 154 and 147 amino acid forms of the proteins in most applications. The 154 AA bFGF protein is more commonly used.

Human bFGF / FGF2 Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant bFGF / FGF2 based upon sequence from: Human
Host: QP5269 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5269. One of the most common applications of our bFGF protein has been in stem cell culture. In stem cell culture, bFGF (along with other nutrients and growth factors) is supplemented in the range of 4-10 ng/ml for systems incorporating feeder cells (eg. HFF or HPF). In systems without feeder cells, much higher concentrations (50-100 ng/ml) of bFGF are recommended.
Bioactivity Data: BioActivity: Concentrations of <5 ng/mL are sufficient to induce cell proliferation of 3T3 cells. The bioactivity acceptance criteria is set at 2 x 105 units/mg.
Monomer or Dimer: Monomer
Amino Acid Sequence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human bFGF / FGF2 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human bFGF / FGF2 Protein

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