Human bFGF / FGF2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant bFGF / FGF2 based upon sequence from: Human
Host: QP5269 protein expressed in E. coli.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5269. One of the most common applications of our bFGF protein has been in stem cell culture. In stem cell culture, bFGF (along with other nutrients and growth factors) is supplemented in the range of 4-10 ng/ml for systems incorporating feeder cells (eg. HFF or HPF). In systems without feeder cells, much higher concentrations (50-100 ng/ml) of bFGF are recommended.
Bioactivity Data: BioActivity: Concentrations of <5 ng/mL are sufficient to induce cell proliferation of 3T3 cells. The bioactivity acceptance criteria is set at 2 x 105 units/mg.
Monomer or Dimer: Monomer
Amino Acid Sequence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Purity: Greater than 95% as determined by SDS-PAGE.
Concentration of Human bFGF / FGF2 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.